Twenty Chemical Element Contents in Normal and Cancerous Thyroid

Thyroid cancer (TC) is the most common endocrine malignancy. TC incidence has dramatically increased in the recent decades [1]. During the same period no other cancer has increased as much as TC. With the worldwide increase in the incidence of TC, it has become the fifth most common cancer in women [2-4]. In some countries, the incidence of TC has increased extremely fast, and it has been the most common cancer for the last years [5].


Introduction
Thyroid cancer (TC) is the most common endocrine malignancy.TC incidence has dramatically increased in the recent decades [1].During the same period no other cancer has increased as much as TC.With the worldwide increase in the incidence of TC, it has become the fifth most common cancer in women [2][3][4].In some countries, the incidence of TC has increased extremely fast, and it has been the most common cancer for the last years [5].
incidence of TC and mortality from this disease increases progressively with advancing age [18,19].For example, a 37-fold increase in hazard ratio from age <40 years to age >70 years was showed in the study of 3664 TC patients that received surgery and adjuvant treatment at Memorial Sloan Kettering Cancer Center from the years 1985 to 2010 [19].
Besides I involved in thyroid function, other chemical elements have also essential physiological functions such as maintenance and regulation of cell function, gene regulation, activation or inhibition of enzymatic reactions, and regulation of membrane function.Essential or toxic (mutagenic, carcinogenic) properties of chemical elements depend on tissue-specific need or tolerance, respectively [20].Excessive accumulation or an imbalance of the chemical elements may disturb the cell functions and may result in cellular degeneration, death or malignant transformation [20][21][22].
In our previous study a significant positive correlation between age and some chemical element contents in the thyroid was observed [23][24][25][26][27][28].It was concluded that an age-dependent excess of intra-thyroidal I and zinc (Zn) concentration are probably one of the factors acting in both initiation and promotion stages of thyroid carcinogenesis [9,24,25], as it was earlier shown by us for I in thyroid and for Zn in prostate gland [29][30][31][32][33][34].Moreover, it seems fair to suppose that besides I and Zn, many other chemical elements also play a role in the pathophysiology of the thyroid.This work had two aims.The first was to assess the silver (Ag), bromine (Br), calcium (Ca), chlorine (Cl), cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), mercury (Hg), I, potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), rubidium (Rb), antimony (Sb), scandium (Sc), selenium (Se), strontium (Sr), and Zn mass fraction contents in TC tissue using three nondestructive instrumental analytical methods: energy dispersive X-ray fluorescent (EDXRF), neutron activation analysis with high resolution spectrometry of short-lived radionuclides (INAA-SLR), and neutron activation analysis with high resolution spectrometry of long-lived radionuclides (INAA-LLR).The second aim was to compare the levels of chemical elements in the malignant thyroid with those in intact (normal) gland of apparently healthy persons.
Normal thyroids for the control group samples were removed at necropsy from 105 deceased (mean age 44±21 years, range 2-87), who had died suddenly.The majority of deaths were due to trauma.A histological examination in the control group was used to control the age norm conformity, as well as to confirm the absence of micro-nodules and latent cancer.
All tissue samples were divided into two portions using a titanium scalpel [35].One was used for morphological study while the other was intended for chemical element analysis.After the samples intended for chemical element analysis were weighed, they were freeze-dried and homogenized [36].
For EDXRF the pounded sample weighing about 8 mg was applied to the piece of Scotch tape serving as an adhesive fixing backing.The content of Br, Cu, Fe, Rb, Sr, and Zn were determined by EDXRF.Details of the relevant facility for this method, source with 109Cd radionuclide, methods of analysis and the results of quality control were presented in our earlier publications concerning the EDXRF analysis of human thyroid and prostate tissue [26,28,[37][38][39].
The pounded samples weighing about 5-10 mg (for biopsy) and 100 mg (for resected materials) were used for chemical element measurement by INAA-SLR.The samples for INAA-SLR were sealed separately in thin polyethylene films washed beforehand with acetone and rectified alcohol.The sealed samples were placed in labeled polyethylene ampoules.The content of Br, Ca, Cl, I, K, Mg, Mn, and Na were determined by INAA-SLR using a horizontal channel equipped with the pneumatic rabbit system of the WWR-c research nuclear reactor (Branch of Karpov Institute, Obninsk).Details of used neutron flux, nuclear reactions, radionuclides, gamma-energies, and spectrometric unit were presented in our earlier publications concerning the INAA-SLR chemical element contents in human thyroid, scalp hair, and prostate [7,23,27,40] In a few days after INAA-SLR all thyroid samples were repacked separately in a high-purity aluminum foil washed with rectified alcohol beforehand and placed in a nitric acid-washed quartz ampoule and used for INAA-LLR.A vertical channel of the WWR-c research nuclear reactor (Branch of Karpov Institute, Obninsk).wasapplied to determine the content of Ag, Co, Cr, Fe, Hg, Rb, Sb, Sc, Se, and Zn by INAA-LLR.Details of used neutron flux, nuclear reactions, radionuclides, gamma-energies, and spectrometric unit were presented in our earlier publications concerning the INAA-LLR chemical element contents in human thyroid, scalp hair, and prostate [24,25,40,41] To determine contents of the elements by comparison with a known standard, biological synthetic standards (BSS) prepared from phenol-formaldehyde resins were used [42].In addition to BSS, aliquots of commercial, chemically pure compounds were also used as standards.For each method ten certified reference material IAEA H-4 (animal muscle) and IAEA HH-1 (human hair) sub-samples were treated and analyzed in the same conditions that thyroid samples to estimate the precision and accuracy of results.
A dedicated computer program for INAA mode optimization was used [43].All thyroid samples were prepared in duplicate, and mean values of chemical element contents were used.Mean values of chemical elements contents were used in final calculation for the Br, Fe, Rb, and Zn mass fractions measured by two methods.Using Microsoft Office Excel, a summary of the statistics, including, arithmetic mean, and standard deviation, standard error of mean, minimum and maximum values, median, percentiles with 0.025 and 0.975 levels was calculated for chemical element contents.The difference in the results between two age groups was evaluated by the parametric Student's t-test and non-parametric Wilcoxon-Mann-Whitney U-test.

Results
Table 1 depicts our data for Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fraction mass fractions in ten sub-samples of IAEA H-4 (animal muscle) and IAEA HH-1 (human hair) certified reference material and the certified values of this material.
The comparison of our results for the Br, Fe, Rb, and Zn mass fractions (mg/kg, dry mass basis) in the normal human thyroid obtained by both EDXRF and INAA methods is shown in Table 2.
Table 3 presents certain statistical parameters (arithmetic mean, standard deviation, standard error of mean, minimal and maximal values, median, percentiles with 0.025 and 0.975 levels) of the Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fraction mass fraction in normal and cancerous thyroid tissue.
The comparison of our results with published data for Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fraction in normal and cancerous thyroid  is shown in Table 4.
The ratios of means and the difference between mean values of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fractions in normal and cancerous thyroid are presented in Table 5.

Twenty Chemical Element Contents in Normal and Cancerous Thyroid
Copyright: © 2018 Vladimir Z, et al.

Twenty Chemical Element Contents in Normal and Cancerous Thyroid
Copyright: © 2018 Vladimir Z, et al.

Discussion Precision and accuracy of results
A good agreement of our results for the Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fractions with the certified values of CRM IAEA H-4 and CRM IAEA HH-1 (Table 1) as well as the similarity of the means of the Br, Fe, Rb, and Zn mass fractions in the normal human thyroid determined by both EDXRF and INAA methods (Table 2) demonstrates an acceptable precision and accuracy of the results obtained in the study and presented in Tables 3-5.
The mean values and all selected statistical parameters were calculated for twenty chemical elements (Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn) mass fractions (Table 3).The mass fraction of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn were measured in all, or a major portion of normal and cancerous tissue samples.

Comparison with published data
Values obtained for Br, Ca, Cl, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, and Zn contents in the normal human thyroid (Table 4) agree well with median of mean values reported by other researches [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55].The obtained means for Ag and Co were almost one order of magnitude lower whereas mean for Sr was almost one order of magnitude higher median of previously reported means but inside the range of means (Table 3).Data cited in Table 3  who died from different non-endocrine diseases.A number of values for trace element mass fractions were not expressed on a dry mass basis by the authors of the cited references.However, we calculated these values using published data for water (75%) [50] And ash (4.16% on dry mass basis) [70] contents in thyroid of adults.
In cancerous tissues (Table 4) our results were comparable with published data for Ca, Cu, Fe, I, Mg, Mn, Rb, Se, and Zn contents.The obtained means for Co, Hg, and Cr were approximately three, two, and one, respectively, order of magnitude lower median of previously reported means and inside the range of these means (Table 4).The obtained mean for Cl was almost one order of magnitude higher the only reported result and mean for K was some higher median of previously reported means and also higher the upper level of the range of these means (Table 4).
No published data referring Ag, Na, Sb, Sc, and Sr contents of cancerous thyroid tissue were found.
The range of means of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn level reported in the literature for normal and for untreated cancerous thyroid vary widely (Table 4).This can be explained by a dependence of trace element content on many factors, including the region of the thyroid, from which the sample was taken, age, gender, ethnicity, mass of the gland, and the cancer stage.Not all these factors were strictly controlled in cited studies.Another and, in our opinion, leading cause of inter-observer variability can be attributed to the accuracy of the analytical techniques, sample preparation methods, and inability of taking uniform samples from the affected tissues.It was insufficient quality control of results in these studies.In many reported papers tissue samples were ashed or dried at high temperature for many hours.In other cases, thyroid samples were treated with solvents (distilled water, ethanol, formalin etc).There is evidence that by use of these methods some quantities of certain trace elements are lost as a result of this treatment That concern not only such volatile halogen as Br, but also other trace elements investigated in the study [71][72][73].

Effect of malignant transformation on chemical element contents
From Table 5, it is observed that in cancerous tissue the mass fraction of Ag, Br, Cl, Cu, and Hg are approximately 13, 9, 2, 3, and 20 times, respectively, higher and also mass fractions of Co, Cr, K, Mg, Na, and Rb are almost in 38%, 55%, 59%, 58%, 28%, and 54%, respectively, higher than in normal tissues of the thyroid.In contrast, the mass fraction of I is almost 26 times lower.Thus, if we accept the chemical element contents in thyroid glands in the control group as a norm, we have to conclude that with a malignant transformation the levels of Ag, Br, Cl, Co, Cr, Cu, Hg, K, Mg, Na, and Rb in thyroid tissue significantly increased whereas the levels of I drastically decrease.

Role of trace elements in malignant transformation of the thyroid
Characteristically, elevated or reduced levels of chemical elements observed in cancerous tissues are discussed in terms of their potential role in the initiation and promotion of thyroid cancer.In other words, using the low or high levels of the chemical element in cancerous tissues researchers try to determine the carcinogenic role of the deficiency or excess of each chemical element in investigated organ.In our opinion, abnormal levels of many chemical elements in tumor could be and cause, and also effect of malignant transformation.From the results of such kind studies, it is not always possible to decide whether the measured decrease or increase in chemical element level in pathologically altered tissue is the reason for alterations or vice versa.

Silver
Ag is a chemical element with no recognized trace metal value in the human body [74].Ag in metal form and inorganic Ag compounds ionize in the presence of water, body fluids or tissue exudates.The silver ion Ag+ is biologically active and readily interacts with proteins, amino acid residues, free anions and receptors on mammalian and eukaryotic cell membranes [75].Besides such the adverse effects of chronic exposure to Ag as a permanent bluish-gray discoloration of the skin (argyria) or eyes (argyrosis), exposure to soluble Ag compounds may produce other toxic effects, including liver and kidney damage, irritation of the eyes, skin, respiratory, and intestinal tract, and changes in blood cells [76].More detailed knowledge of the Ag toxicity can lead to a better understanding of the impact on human health, including thyroid function.Anyway, a drastically elevated level of Ag in malignant thyroid tumors could possibly be explored for diagnosis of TC.

Bromine
This is one of the most abundant and ubiquitous of the recognized trace elements in the biosphere.Inorganic bromide is the ionic form of bromine which exerts therapeutic as well as toxic effects.An enhanced intake of bromide could interfere with the metabolism of iodine at the whole-body level.In the thyroid gland the biological behavior of bromide is more similar to the biological behavior of iodide [77].
In our previous studies, we found a significant age-related increase of Br content in human thyroid [23,[26][27][28].Therefore, a goitrogenic and, probably, carcinogenic effect of excessive Br levels in the thyroid of old females was assumed.On the one hand, elevated levels of Br in TC tissues, observed in the present study, supports this conclusion.But, on the other hand, bromide compounds, especially potassium bromide (KBr), sodium bromide (NaBr), and ammonium bromide (NH4Br), are frequently used as sedatives in Russia [78].It may be the reason for elevated levels of Br in specimens of patients with TC.Nevertheless, the accumulation of Br in neoplastic thyroid tissues could possibly be explored for diagnosis of TC.

Chlorine
Cl is a ubiquitous, extracellular electrolyte essential to more than one metabolic pathway.Cl exists in the form of chloride in the human body.In the body, it is mostly present as sodium chloride.Therefore, as usual, there is a correlation between Na and Cl contents in tissues and fluids of human body.It is well known that Cl mass fractions in samples depend mainly on the extracellular

Twenty Chemical Element Contents in Normal and Cancerous Thyroid
Copyright: © 2018 Vladimir Z, et al.
water volume, including the blood volumes, in tissues [79].Cancerous tissues are predominantly highly vascularized lesions [80][81][82][83][84][85].Thus, it is possible to speculate that thyroid malignant tumors are characterized by an increase of the mean value of the Cl mass fraction because the level of tumor vascularization is higher than that in normal thyroid tissue.Overall, the elevated levels of Cl in neoplastic thyroids could possibly be explored for diagnosis of TC.

Cobalt
Health effects of high Co occupational, environmental, dietary and medical exposure are characterized by a complex clinical syndrome, mainly including neurological, cardiovascular and endocrine deficits, including hypothyroidism [86,87].Co is genotoxic and carcinogenic, mainly caused by oxidative DNA damage by reactive oxygen species, perhaps combined with inhibition of DNA repair [88].In our previous studies it was found a significant age-related increase of Co content in female thyroid [25].Therefore, a goitrogenic and, probably, carcinogenic effect of excessive Co level in the thyroid of old females was assumed.Elevated level of Coin TC tissues, observed in the present study, supports this conclusion.Anyway, the accumulation of Co in malignant thyroid tumors could possibly be explored for diagnosis of TC.

Chromium
Cr-compounds are cytotoxic, genotoxic, and carcinogenic in nature.Some Cr forms, including hexavalent chromium (Cr6+), are toxicants known for their carcinogenic effect in humans.They have been classified as certain or probable carcinogens by the International Agency for Research on Cancer [89].The lung cancer risk is prevalent in pigment chromate handlers, ferrochromium production workers, stainless steel welders, and chrome-platers [90].Except in Cr-related industries and associated environments, Cr intoxication from environmental exposure is not common.However, it was found, that drinking water supplies in many geographic areas contain chromium in the +3 and +6 oxidation states.Exposure of animals to Cr6+ in drinking water induced tumors in the mouse small intestine [91].Many other animal experiments and in vitro studies demonstrate also that Cr can induce oxidative stress and exert cytotoxic effects [92].Besides reactive oxygen species (ROS) generation, oxidative stress, and cytotoxic effects of Cr exposure, a variety of other changes like DNA damage, increased formation of DNA adducts and DNA-protein cross-links, DNA strand breaks, chromosomal aberrations and instability, disruption of mitotic cell division, chromosomal aberration, premature cell division, S or G2/M cell cycle phase arrest, and carcinogenesis also occur in humans or experimental test systems [90].Anyway, the accumulation of Cr in malignant thyroid tumors could possibly be explored for diagnosis of TC.

Copper
Cu is a ubiquitous element in the human body which plays many roles at different levels.Various Cu-enzymes (such as amine oxidase, ceruloplasmin, cytochrome-c oxidase, dopamine-monooxygenase, extracellular superoxide dismutase, lysyl oxidase, peptidylglycineamidating monoxygenase, Cu/Zn superoxide dismutase, and tyrosinase) mediate the effects of Cu deficiency or excess.Cu excess can have severe negative impacts.Cu generates oxygen radicals and many investigators have hypothesized that excess copper might cause cellular injury via an oxidative pathway, giving rise to enhanced lipid per oxidation, thiol oxidation, and, ultimately, DNA damage [93][94][95].Thus, Cu accumulation in thyroid parenchyma with age may be involved in oxidative stress, dwindling gland function, and increasing risk of goiter or cancer [26,28].The significantly elevated level of Cu in thyroid malignant tumors, observed in the present study, supports this speculation.However, an overall comprehension of Cu homeostasis and physiology, which is not yet acquired, is mandatory to establish Cu exact role in the thyroid malignant tumors etiology and metabolism.Anyway, the accumulation of Cu in neoplastic thyroids could possibly be explored for diagnosis of TC.

Mercury
Hg is one of the most dangerous environmental pollutants [96].The growing use of this metal in diverse areas of industry has resulted in a significant increase of environment contamination and episodes of human intoxication.Hg damages the central nervous system and has irreparable effects on the kidneys [97].Hg may also harm a developing fetus and decrease fertility in men and women [98].Besides these effects, Hg has been classified as certain or probable carcinogen by the International Agency for Research on Cancer [89].For example, in Hg polluted area thyroid cancer incidence was almost 2 times higher than in adjacent control areas [99].
Negative effects of Hg are due to the interference of this metal in cellular signaling pathways and protein synthesis during the period of development.Since it bonds chemically with the sulfur hydride groups of proteins, it causes damage to the cell membrane and decreases the amount of RNA [100].Moreover, it was shown that Hg may be involved in four main processes that lead to genotoxicity: generation of free radicals and oxidative stress, action on microtubules, influence on DNA repair mechanisms and direct interaction with DNA molecules [101].Anyway, a drastically elevated level of Hg in malignant thyroid tumors could possibly be explored for diagnosis of TC.

Iodine
Compared to other soft tissues, the human thyroid gland has higher levels of I, because this element plays an important role in its normal functions, through the production of thyroid hormones (thyroxin and triiodothyronine) which are essential for cellular oxidation, growth, reproduction, and the activity of the central and autonomic nervous system.Malignant transformation is accompanied by a loss of tissue-specific functional features, which leads to a significant reduction in I content associated with functional characteristics of the human thyroid tissue.Drastically low level of I content in neoplastic thyroids could possibly be explored for diagnosis of TC.

Potassium
An uncontrollable cell proliferation characterize the malignant tumors.Therefore, morphological structures of TC tissue differ from the structure of normal thyroid parenchyma.Because K is mainly an intracellular electrolyte, an elevated level of K content in the TC tissue might reflect increase of ratio "mass of transformed thyroid cell -mass of follicular colloid".Nevertheless, the accumulation of K in neoplastic thyroids could possibly be explored for diagnosis of TC.

Magnesium
Mg is abundant in the human body.This element is essential for the functions of more than 300 enzymes (e.g.alkaline phosphatase, ATP-ases, phosphokinases, the oxidative phosphorylation pathway).It plays a crucial role in many cell functions such as energy metabolism, protein and DNA syntheses, and cytoskeleton activation.Moreover, Mg plays a central role in determining the clinical picture associated with thyroid disease [102].Experimental data have shown that high doses of magnesium increase the activity of the thyroid gland [103].Magnesium deficiency can influence bioavailability and tissue distribution of selenium which then appears diminished [104].From these data, one can conclude that Mg is involved in the thyroid function.If so, significant reduction in Mg content cam be associate with TC, because malignant transformation is accompanied by a loss of thyroid-specific functional features.However, it is well known that malignant tumors have an usually higher Mg levels than do normal tissues [105][106][107][108][109][110], possibly caused by the "retention" of Mg by the tumor [111], as a result of the high Mg requirement of growing cells.In addition, cultured proliferating cells have long been known to contain more magnesium than quiescent cells, and experimental conditions that decreased magnesium availability affected cell proliferation rate [112].Thus, the elevated levels of Mg in neoplastic thyroids could possibly be explored for diagnosis of TC.

Sodium
Knowledge concerning ion regulation in many normal and abnormal cell processes has had a rapid development.It was found, among other regulations, that sodium-calcium exchange is associated with the cytoskeleton and the cell membrane.A hypothesis was eventually established that a wide variety of pathological phenomena ranging from acute cell death to chronic processes, such as neoplasia, all have a common series of cellular reactions [113].In accordance with this hypothesis, concentrations of sodium were found to be enhanced in human and animal neoplastic tissues [114,115].Moreover, the hypothesis that physiological and biochemical changes associated with proliferating malignant tumors may cause an increase in total tissue sodium concentration was tested with non-invasive, quantitative 23Na magnetic resonance imaging in patients with benign and malignant breast tumors.It was shown that elevated Na concentrations in breast lesions appears to be a cellularlevel indicator associated with malignancy [116].In addition, Na is mainly an extracellular electrolyte and its elevated level in malignant tumors might link with a high tumor vascularization (see Chlorine).Anyway, it seems that the accumulation of Na is a generic property of malignant tumors.

Rubidium
As for Rb, there is very little information about its effects in organisms.No negative environmental effects have been reported.Rb is only slightly toxic on an acute toxicological basis and would pose an acute health hazard only when ingested in large quantities [117].Rb has some function in immune responce [118], probably by supporting cell differentiation [119].Potassium (K) and Rb are in the first group of the periodic table.Rb, like K, seems to be concentrated in the intracellular space and transfered through membrane by the Na+K+-ATPase pump.An overload of Rb could modulate proliferative responses of the cell, as was shown for bone marrow leukocytes [78].In our previous studies it was found a significant age-related increase of Rb content in female thyroid [25,28].Therefore, a goitrogenic and, probably, carcinogenic effect of excessive Rb level in the thyroid of old females was assumed.Elevated level of Rb in TC tissues, observed in the present study, supports this conclusion.Anyway, the accumulation of Rb in malignant thyroid tumors could possibly be explored for diagnosis of TC.
Our findings show that mass fraction of Ag, Br, Cl, Co, Cr, Cu, Hg, I, K, Mg, Na, and Rb are significantly different in TC as compared to normal thyroid tissues (Tables 5).Thus, it is plausible to assume that levels of these chemical elements in thyroid tissue can be used as tumor markers.However, this subjects needs in additional studies.

Limitations
This study has several limitations.Firstly, analytical techniques employed in this study measure only twenty element (Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn) mass fractions.Future studies should be directed toward using other analytical methods which will extend the list of chemical elements investigated in normal and cancerous thyroid tissue.Secondly, the sample size of TC group was relatively small.It was not allow us to carry out the investigations of chemical element contents in TC group using differentials like gender, histological types of tumors, stage of disease, and dietary habits of healthy persons and patients with TC.Lastly, generalization of our results may be limited to Russian population.Despite these limitations, this study provides evidence on cancer-specific tissue Ag, Br, Cl, Co, Cr, Cu, Hg, I, K, Mg, Na, and Rb level alteration and shows the necessity the need to continue chemical element research of malignant thyroid tumors.

Conclusion
In this work, chemical elemental measurements were carried out in the tissue samples of normal thyroid and malignant tumors of thyroid using three non-destructive instrumental analytical methods: EDXRF, INAA-SLR, and INAA-LLR.It was shown that the combination of these methods is an adequate analytical tool for the non-destructive determination of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn content in the tissue samples of human thyroid, including needle-biopsy cores.It was observed that in cancerous tissues content of Ag, Br, Cl, Co, Cr, Cu, Hg, K, Mg, Na, and Rb significantly increased whereas the levels of I drastically decrease in a comparison with the normal thyroid tissues.In our opinion, the increase in levels of Ag, Br, Cl, Co, Cr, Cu, Hg, K, Mg, Na, and Rb, as well as the decrease in levels of I in cancerous tissue might demonstrate an involvement of these elements in etiology and pathogenesis of malignant thyroid tumors.It was supposed that the changes in levels Ag, Br, Cl, Co, Cr, Cu, Hg, I, K, Mg, Na, and Rb in thyroid tissue can be used as tumor markers.
also includes samples obtained from patients Citation: Vladimir Z, Sofia Z (2018) Twenty Chemical Element Contents in Normal and Cancerous Thyroid.Int J Hematol Blo Dis 3(2).

Element IAEA H-4 animal muscle This work results IAEA HH-1 human hair This work results
EDXRF, INAA-SLR and INAA-LLR data of chemical element contents in certified reference material IAEA H-4 (animal muscle) and IAEA HH-1 (human hair) compared to certified values (mg/kg, dry mass basis) Table1:

Table 2 :
Comparison of the mean values (M±SEM) of the chemical element mass fractions (mg/kg, dry mass basis) in the normal human thyroid obtained by both EDXRF and INAA methods M -Arithmetic mean, SEM -standard error of mean.

Table 3 :
Some statistical parameters of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fraction (mg/kg, dry mass basis) in normal and cancerous thyroid M -arithmetic mean, SD -standard deviation, SEM -standard error of mean, Min -minimum value, Max -maximum value, P 0.025 -percentile with 0.025 level, P 0.975 -percentile with 0.975 level.

Table 4 :
Median, minimum and maximum value of means Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn contents in the normal and cancerous thyroid according to data from the literature in comparison with our results (mg/kg, dry mass basis)

Table 5 :
Differences between mean values (M±SEM) of Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn mass fraction (mg/ kg, dry mass basis) in normal and cancerous thyroid M -arithmetic mean, SEM -standard error of mean, Statistically significant values are in bold.