Case Report Open Access
48, XXYY Genotype: Case Report of a Rare Clinical Syndrome
Arsha Sreedhar1*, Deborah Feden1, Gretchen Perilli2, Ranjit Nair3, VasudevMagaji2
1Department of Internal Medicine, Lehigh Valley Health Network, Allentown, PA 18101, USA
2Department of Endocrinology, Lehigh Valley Health Network, Allentown, PA 18103, USA
3Department of Hematology and Oncology, Lehigh Valley Health Network, Allentown, PA 18103, USA
*Corresponding author: Arsha Sreedhar, Department of Internal Medicine, Lehigh Valley Health Network, One City Center, 707 Hamilton Street, 9th Floor Allentown, PA 18101, USA, Tel: +484-862-3159; Fax: +484-862-3175; E-mail: @
Received: June 11, 2015; Accepted: December 17, 2015; Published: January 01, 2016
Citation: Sreedhar A, Feden D, Perilli G, Nair R, Magaji V (2015) 48, XXYY Genotype: Case Report of a Rare Clinical Syndrome. J Endocrinol Diab 3(1): 1-3. DOI:
48, XXYY syndrome is extremely rare with an incidence of 1:18000-1: 100,000 males. Classic Klinefelter Syndrome (KS) with 47, XXY karyotype has well recognized clinical features which includes seminiferous tubule dysgenesis, androgen deficiency, cognitive and behavioral dysfunction, increased risk of autoimmune disorders, thromboembolic disease and cancer. However the clinical features of 48, XXYY syndrome are extremely diverse and more pronounced in comparison to classic KS. With each additional X chromosome there is progressive divergence from the normal with increased frequency of phenotypic, endocrine and structural abnormalities. We describe here the case of a 28-year old male patient, who was diagnosed with classic KS at a very young age of 14, presented with not only behavioral and cognitive impairment but also with a wide spectrum of medical complications. His distinct clinical features led a comprehensive re-evaluation with repeat karyotyping and was identified as XXYY genotype.
Klinefelter Syndrome (KS) is the most common sex chromosomal disorder which occurs in approximately 1 per 500 live born male births [1]. The characteristic clinical features of KS with the classic 47, XXY karyotype is well recognized which includes seminiferous tubule dysgenesis, androgen deficiency, cognitive and behavioral dysfunction, increased risk of autoimmune disorders, thromboembolic disease and cancer [2]. With each additional X chromosome there is progressive divergence from the normal with increased frequency of phenotypic, endocrine and structural abnormalities [3]. Poly X-syndrome, often described in literature as a variant of KS, is much rarely reported with a prevalence of 1:18,000-1:100,000 male births and has more pronounced congenital malformations and associated medical comorbidities [4]. We describe a case of a patient who had a formal diagnosis of classic KS from a very young age of 14, presented with not only behavioral and cognitive impairment but also with a wide spectrum of medical complications and further identified on reassessment as XXYY genotype.
A 28-year-old male with a known diagnosis of KS presented to our office for a routine visit. The diagnosis of classic KS was initially made during a learning disability evaluation at the age of 14. Intriguingly his medical history was very complicated for his very young age.

At age 13 he was diagnosed with common variable immunodeficiency and had recurrent upper respiratory infections until the initiation of immunoglobulin therapy. He suffered from childhood asthma with severe restrictive ventilatory defect on pulmonary function test. His other co-morbidities at that time included type 2 diabetes, metabolic syndrome with evidence of moderate sleep apnea. He had history of very low testosterone level with previous testosterone replacement however with he has been extremely non-compliant to treatment. He suffered NSTEMI at the age of 15 for which he has been on aspirin. His recent echocardiogram showed preserved ejection fraction, severe left ventricular hypertrophy and hypokinesis of the basal inferoseptum consistent with his history of myocardial infarction. By the age of 16, he developed 3 episodes of unprovoked Deep Vein Thrombosis (DVT) and further an episode of acute embolic cerebrovascular accident. Subsequent evaluation with transesophageal echocardiogram showed septum Secundum Atrial Septal Defect (ASD) with Patent Foramen Ovale (PFO) which contributed to the paradoxical embolic event. Due to multiple cardiovascular events he underwent surgical repair of PFO. His multiple DVTs despite anticoagulation prompted IVC filter insertion.

He has significant psychiatric history including bipolar disorder with multiple hospitalizations for agitation, impulsive behavior, auditory hallucinations and suicidal ideations. His diagnosis included oppositional defiant disorder, learning disability and mild mental retardation with an IQ of 62. The patient was on maintenance dose of lithium which required discontinuation at a later period secondary to an episode of renal failure. Subsequently he has been on high dose of quetiapine and citalopram. He continued to have outbursts and periods of agitation lasting for minutes but was easily redirectable.

His current physical examination revealed a tall stature with a height 76.8 inch and weighed 166 kilogram. He had sparsefacial and body hair, female fat distribution, firm atrophic testis and bilateral gynecomastia. CT imaging studies of head showed underdeveloped left frontal sinus with poor dentition (Figure 1). Cervical spine imaging showed loss of cervical lordosis (Figure 2). Plain radiography showed congenital dysplastic anomaly of right radial head (Figure 3).

Due to his multiple diverse medical problems, a karyotypic analysis was repeated at our office which revealed 48, XXYY genotype.
KS is named after Harry Klinefelter, who first described the syndrome in 1942 [5,6]. In 1959, Jacobs, et al. [7] described XXY as the chromosomal constitution of KS. KS results from extra X chromosome in 80% of cases while in the remaining cases
Figure 1: Craniofacial abnormalities-CT image showing underdeveloped left frontal sinus with poor dentition.
Figure 2: Cervical spine imaging showed loss of cervical lordosis.
Figure 3: Congenital deformity of head of radius..
this arises from numerous sex chromosome abnormalities (48, XXXY, 48, XXYY, 49, XXXXY) or 46, XY/ 47, XXY mosaicism, or structurally abnormal sex chromosomes [8] .

Due to distinguishable symptoms, physical features and multiple long term complications 48, XXYY syndrome should be considered a distinct clinical syndrome. 48, XXYY syndrome is extremely rare with an incidence of 1:18000 - 1: 100,000 males [4,9-11]. 48, XXYY syndrome is usually caused by double non-disjunction occurring at both the first and second meiotic division during spermatogenesis and fusion of the resulting 25, XYY spermatozoon with a 23, X ovum, or nondisjunction at the second meiotic division in both parents resulting in the fusion between a 24, XX ovum and a 24, YY sperm [2]. It has been studied that in poly-X KS: 48, XXXY and 49, XXXXY, that the additional sex chromosomes almost always arise from a single parent, usually the mother while in 48, XXYY it is paternal in origin [4] .

The physical features of 48, XXYY syndrome are similar to 47, XXY but with more pronounced phenotypic abnormalities including craniofacial dysmorphism, skeletal anomalies such as radioulnar, synostosis and clinodactyly, lower cognitive function resulting in developmental delays, congenital cardiac anomalies, hypogonadism and asthma manifesting at younger age [1,9]. 48, XXYY patients are often tall with an eunuchoid habitus with adult height above 6 feet [1,9]. Other common clinical features include sparse body hair, small testicles and penis and gynecomastia. Peripheral vascular disease may result in leg ulcers and varicosities. There is a reported higher rate of hospitalizations among Poly X syndrome patients for respiratory infections and asthma exacerbations pointing towards immunological dysfunctions and subsequent infections [9].

There is increased reported incidence of congenital heart disease reported in 48, XXYY syndrome which includes septal defect, tetralogy of fallot, pulmonary stenosis, mitral valve prolapsed [9]. Our patient had septum secundum ASD which accounted for the paradoxical cerebrovascular accident.

Autism spectrum disorders have been reported in more than 1/3rd and ADHD in 70% of 48, XXYY syndrome [4,12].

Their behavior abnormalities include hyperactivity, attention problems, impulsivity, aggression, mood instability, autistic-like behaviors, and poor social function [9]. In classic KS, the typical cognitive pattern shows deficit in specific domains of cognition including language and frontal-executive functions, rather than a general decline in intellectual ability [13]. 48, XXYY males have significantly lower IQ and language disability than classic 47, XXY with studies showing an IQ reduction of 15-16 points with every additional X chromosome [1,14]. They are usually shy however can be aggressive and impulsive with significant academic, social and behavioral deficits [1]. 48, XXYY males have significantly lower verbal IQ (VIQ) when compared to Performance IQ (PIQ) and interestingly the VIQ decreases with age while PIQ remains mostly unchanged [12]. As a result 48, XXYY males have significantly lower language associated disability and adaptive functioning than males with classic 47, XXY [1,4].

Our patient had DVT at a very early age of 16 with multiple recurrences. There is increased incidence of DVT in 48, XXYY syndrome affecting more than 20% over the age of 20 years. The pathophysiology this not been elucidated but could be attributed to estrogen testosterone imbalance [4]. Type II diabetes is seen in about 18% of patients with KS 48, XXYY. Hypergonadotropic hypogonadism is common in pubertal-aged boys which would require treatment with testosterone replacement therapy [9]. Behavioral issues in these groups of patients can make the management of these extremely challenging. The association of classic KS, 47, XXY with malignant diseases is well known. An analysis of cancer incidence by karyotype revealed that men with 48, XXYY had a particularly elevated incidence of non-Hodgkin lymphoma, but due to the rarity of this syndrome there is no conclusive data on the incidence of specific malignancies in 48, XXYY [15].

Medical work up at the time of diagnosis should include a baseline evaluation including comprehensive neurodevelopmental assessment, a cardiac evaluation for congenital heart defects, renal ultrasound due to association with unilateral kidney and orthopedic evaluation for skeletal abnormalities. Other test should be considered based on clinical symptoms. Adolescents and adults should also be screened diabetes, hypothyroidism, hypogonadism, osteoporosis, and obesity. All patients need to be screened for autism spectrum disorders, ADHD, mood disorders and other mental health problems. We underscore the importance of having a high index of suspicion for deep vein thrombosis, acute coronary syndrome and non-Hodgkin lymphoma based on clinical symptoms [9]. Management should include a multidisciplinary approach including experts in endocrinology, psychiatry, neurology, speech/language therapy, occupational therapy, and physical therapy.
This case presents important comparisons between the phenotypes of 48, XXYY syndrome and the classic 47, XXY karyotype. Early recognition, through neurodevelopment assessment and close long term follow up is critical in patients with 48, XXYY syndrome due to its multiple medical, psychosocial and life threatening associations.
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