Research Article Open Access
Novel Synthesis and Characterization of 2-Aminobenzimidazole Derivative
R Sriram1, R Sapthagiri1, A Ravi2*
1Research Scholars, PG & Research Department of Chemistry, Government Arts College, Tiruvannamalai-606603, Tamil Nadu, India
2Assistant professor, PG & Research Department of Chemistry, Government Arts College, Tiruvannamalai-606603, Tamil Nadu, India
*Corresponding author: A Ravi, Assistant professor, PG & Research Department of Chemistry, Government Arts College, Tiruvannamalai-606603, Tamil Nadu, India, E-mail:
Received: June 30, 2015; Accepted: July 14, 2015; Published: July 30, 2015
Citation: Sriram R, Sapthagiri R, Ravi A (2015) Novel Synthesis and Characterization of 2-Aminobenzimidazole Derivative. SOJ Mater Sci Eng 3(2): 1-4.
An efficient method for the synthesis of 2-aminobenzimidazole derivative using iodoacetic acid mediated cyclization of thiourea. This method is also applicable to the synthesis of more challenging derivative such as 7-(methanesulphonaminomethyl)-N-(1-(3- (trifluoromethyl) phenyl) ethyl)-1H-benzo[d]imidazol-2-amine. The method was simpler and giving high yield of the target molecule.

Keywords: 3-methylbenzene-1,2-diamine; 1-(3-(trifluoromethyl) phenyl)ethanamine; NBS; Raney nickel; Methanesulphonylchloride; Iodoacetic acid; N-((2-(1-(3-(trifluoromethyl)phenyl)ethylamino)- 1H-benzo[d]imidazol-4-yl) methyl) methanesulfonamide
Generally benzimidazole derivatives in numerous categories of therapeutic agent such as antimicrobial, antiviral, anti inflammatory, antioxidant, anticancer, anticoagulant, liquid level modulator, antidiabetics etc., various substituted around the benzimidazole nucleus have provided a wide biological activities. Important of this nucleus we are synthesized novel 2-aminobenzimidazole such as like N-((2-(1-(3-(trifluoromethyl) phenyl) ethylamino)-1H-benzo[d]imidazol-4-yl) methyl) methanesulfonamide. Aminobenzimidazoles are interesting heterocycles that are found in natural products as well as drugs and number of biologically active molecules [1]. The synthesis of 2-aminobenzimidazole involves the cyclodesulfurization of a pre-formed thiourea. The reported desulfurization agents was mercury(II) oxide [2], mercury(II) chloride [3], copper(I) chloride [4], methyl iodide [5], tosyl chloride [6], dicyclohexyl carbodiimide [7], PhI(OAc)2 [8] and polymer supported carbodiimide [9] etc., the reported method was frequently required heating and long reaction time. Furthermore formation of urea side product can pose significant challenges during purification. The variety of 2-aminobenzimidazole derivatives synthesized from diverse isothiocyanates (electron donating and electron withdrawing) groups was well tolerating the reaction.

We recognized that this process could be considerably simplified by using iodoaceic acid as a cyclodesulfurization agent and using ethanol solvent to provide desired product at 60°C, 3 hours without formation of side product with high yield.
The chemicals of 3-methylbenzene-1,2-diamine, 1-(3-(trifluoromethyl) phenyl) ethanamine, NBS, methanesulphonylchloride, iodoacetic acid, thionyl chloride were obtained from Avra chemicals and Raney nickel was purchased from Aldrich. Silica gel (TLC and Column grade) were purchased from Merck.

FTIR spectra (KBr pellets) were measured using Alpha Bruker FTIR instrument scanning with the entire region of 4000 - 400 cm-1 with typical resolution of 1.0 cm-1. The NMR spectra of the compounds have been recorded on Bruker AV400 spectrometer operating at 400 MHz for recording 1H NMR spectra in CDCl3 as solvent using TMS as internal standard. Mass spectra have been recorded on SHIMADZU spectrometer using chemical ionization technique.
Preparation of 7-(methanesulphonaminomethyl)-N-(1- (3-(trifluoromethyl)phenyl)ethyl)-1H-benzo[d]imidazol- 2-amine: To a solution of N-(2,3-diaminobenzyl)methanesulfonamide (1) (3g, 0.0139mmol, 1eq), 1-(trifluoromethyl)-3-(1- isothiocyanatoethyl)benzene (2) (3.2g, 0.0139 mmol, 1eq) and iodoacetic acid (1.7g, 0.0139mmol, 1eq) in 30 mL of anhydrous ethanol was added at 0°C. The reaction mixture was heated at 60°C and stirred for 3 hrs. TLC was indicated absence of N-(2,3- diaminobenzyl)methanesulfonamide. The residue was diluted with water and then extracted with Dichloro methane (30 ml* 3times). The combined organic layer washed with brine solution and dried with anhydrous sodium sulphate and filtered, concentrated in vacuum to afford crude product. The crude was purified by (60-120mesh silica gel) chromatography column using chloroform, ethanol as eluvent (90:10) to get pure desired product as ethyl 7-(methanesulphonaminomethyl)-N-(1-(3- (trifluoromethyl)phenyl)ethyl)-1H-benzo[d]imidazol-2-amine (3) (3.5g,) with 92% yield.
Results and Discussion
Spectral details of 7-(methanesulphonaminomethyl)-N- (1-(3-(trifluoromethyl) phenyl)ethyl)-1H-benzo[d]imidazol- 2-amine IR (film): 3332 (NH), 3365 (NH) and Aromatic C-H (2966) cm-1 Mass (m/z): Calculated M.W: 413.0, Observed M.W: 414.0 (M+1 (Figure 1)

1H NMR (300 MHz, DMSO –d6): δ = 10.94 (s, 1H -NH), 7.81 (s, 1H -NH), 7.74(s, 1H, -NH) 7.57(s, 1H, Ar-H), 7.53(d, 1H, J = 9 Hz, Ar-H), 7.315-7.575(m, 2H, Ar-H), 7.03(d,1H, J = 7.5Hz, Ar- H),6.92(d, 1H, J = 6.9Hz, Ar-H), 6.84 -6.83(d, 1H, J = 5.7Hz, Ar-H), 5.06 (m, 1H, Ali-CH),4.33(broad s, 2H, -CH2), 2.75(s, 3H, -CH3), 1.50(d, 3H, J = 6.9Hz, -CH3) (Figure 2).

13C NMR (100.6MHz, DMSO d-6): δ = 169.0, 155.1, 147.7, 130.7, 129.7, 129.5, 129.2, 126.1, 123.8, 122.8, 120.3, 113.6, 106.3, 72.0, 59.0, 51.7, 42.5, 23.9 (Figure 3) Figure (1-3) revealed the Mass, 1H NMR and 13C NMR spectra of 7-(methanesulphonaminomethyl)-N-(1-(3-(trifluoromethyl) phenyl)ethyl)-1H-benzo[d]imidazol-2-amine (3) respectively using compound 1 and 2 with ethanol as solvent in the presence of iodoacetic acid has been shown in the scheme 1.
Figure 1: MASS Spectrum of the Compound.
Figure 2: 1H NMR Spectrum of the Compound.
Figure 3: 13C NMR Spectrum of the Compound.
Scheme 1: Spectrum of compound 3.
FTIR spectra of compound 3 have been provided a preliminary idea for the formation of product. According to the FTIR, the presence of peak at 3480 cm-1 has clearly noticed the utilization of starting materials transforms into the product. Further, the corresponding peaks at 3332, 3365 and 2966 cm-1 have been related to aliphatic N-H, benzimidazole ring N-H, C-H aromatic stretching in the compound 3. The concerned mass of the compound 3 is in good agreement with the observed (414.0 m/z) and calculated value (413.0 m/z) were shown in Figure 1 Proton NMR strongly empowered for the formation of the product by its δ value. In proton NMR the three exchangeable –NH protons showing peaks at δ(ppm) = 10.94 (s, 1H), 7.81 (s, 1H), 7.74(s, 1H,). Four aromatic doublet protons gives peak at δ=7.03(d,1H, J= 7.5Hz, Ar-H), 6.92(d, 1H, J = 6.9Hz, Ar-H), 6.82(d, 1H, J = 5.7Hz, Ar- H), aromatic multiplet protons provide peak at δ=7.315-7.575(m, 2H, Ar-H), methane proton appeared at 5.06 (m, 1H, Ali-CH) sulphonamide methylene protons showing peak at 4.33(broad s, 2H, -CH2), and two methyl proton indicate peak at 2.75(s, 3H, -CH3), 1.50(d, 3H, J = 6.9Hz, -CH3) ppm. Also 13C NMR spectra were provide peaks at d = 169.0ppm which shows impurity peak of iodoacetic acid carbonyl carbon, aromatic carbons are absorbed the range of δ = 155.1, 147.7, 130.7, 129.7, 129.5, 129.2, 126.1, 123.8, 122.8, 120.3, 113.6, 106.3, 72.0. Methine carbon, sulphonamide methylene and methyl, aliphatic methyl group carbon gives peaks at 59.0, 51.7, 42.5, 23.9 respectively. These values confirmed to the number of carbon atoms present in the compound
In the present work 7-(methanesulphonaminomethyl)-N- (1-(3-(trifluoromethyl)phenyl)ethyl)-1H-benzo[d]imidazol-2- amine (3) were synthesized successfully from the condensation between compound 1 and 2 in the presence of iodoacetic acid. Most of the researcher have been synthesized 2-aminobenzimidazole derivatives by using hazardous catalyst like mercury(II)oxide, mercury(II)chloride, copper(I) chloride, methyl iodide etc., which involves long time reaction and significant challenges during purification of the products. we have been synthesized the 2-aminobenzimidazole derivatives considerably simplified method used by iodoacetic acid as a cyclode sulfirization agent and using ethanol as solvent to provide desired product at 60°C, 3 hours without formation of side product with high yield.

In our scheme synthesis of compound 1 was involved 6 steps. The first step was protection of diamine followed by bromination and Di BOC amination, BOC deprotection, sulfonation using methane sulfonyl chloride and deprotection of diamine using raney nickel to get key intermediate 1 and 2 was synthesized using amine and thiophosgene. The chemical structures of compound 3 have been confirmed using various spectral techniques viz., FTIR, Mass, 1H-NMR and 13C spectra and were found to be in agreement with the chemical structures expected.

In literature survey variety of 2-aminobenzimidazole derivatives were reported using 3,4-substituted diamines and para trifluoro substituted isothiocynates as a key starting material. These derivatives show wide range of biological activity. So in our work we are synthesized various novel 2,3 substituted diamine derivative like compound (1) and various meta trifluoro substituted isothiocynates like compound (2). By using those key intermediates we are synthesized various substituted 2-aminobenzimidazole like N-((2-(1-(3-(trifluoromethyl) phenyl) ethylamino)-1Hbenzo[ d]imidazol-4-yl)methyl)methanesulfonamide(3) which can possibly be further modified to research better potency than the reference drugs.
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