Research Article
Open Access
Comparative Study on the Efficacy of Amprolium and
Sulfadimidine in Coccidia Infected Chickens in Debre-
Zeit Agricultural Research Center Poultry Farm,
Bishoftu, Ethiopia
Abdisa Hunduma1, Bedaso Kebede1*
1Veterinary Drug and Animal Feed Administration and Control Authority, Ministry of Livestock and Fisheries
*Corresponding author: Bedaso Kebede, Veterinary Drug and Animal Feed Administration and Control Authority, Ministry of Livestock and Fisheries,
Ethiopia; E-mail:
@
Received: 30 September 2016; Accepted: 17 October 2016; Published:27 October 2016
Citation: Hunduma A, Kebede B (2016) Comparative Study on the Efficacy of Amprolium and Sulfadimidine in Coccidia Infected Chickens in Debre-Zeit Agricultural Research Center Poultry Farm, Bishoftu, Ethiopia. SOJ Vet Sci 2(2): 1-5. DOI: 10.15226/2381-2907/2/2/00121
Abstract
An experimental type of study was conducted to assess the
efficacy of Amprolium and Sulfadimidine drugs in the treatment
of chickens’ coccidiosis at Debre-zeit Agricultural Research Center
poultry farms. Total 52 chickens were purposively selected for this
study. Among them 17 chickens were used for control group while
35 chickens were used for treatment group. The thirty five chickens
used for treatment group were 18 of them treated with Amprolium
and the rest 17 were treated with Sulfadimidine. The drugs were
administered orally in drinking water. The treatment response of
these drugs for coccidiosis with respect to reduction in fecal scores
(OPG count) was done by comparing OPG count before and after
treatment. This fecal Oocyst count (OPG count) was conducted by
using the modified McMaster Oocyst count. The mean OPG count
between before treatment and after treatment was 3288.8 and
1468.65 respectively. This result denotes that the OPG count after
treatment was not significantly reduced (p > 0.05) when compared
with OPG count before treatment. The OPG count between before
and after treatment was statistically significant difference (p <
0.05). The current findings of the study showed as there was no
statistically significant difference in OPG count between before and
after treatment in Amprolium treated groups (p > 0.05), but in the
Sulfadimidine administered group, there was statistically significant
difference (p=0.004). This reveals that Sulfadimidine is more effective
than Amprolium to treat coccidial infection. Adequate measures must
be taken to avoid coccidiosis outbreaks; otherwise it will continue to
cause considerable losses to the poultry industry particularly when
favorable conditions for the parasite development are created.
Keywords: Amprolium; Coccidian; Eimeria; OPG Count; Sulfadimidine
Keywords: Amprolium; Coccidian; Eimeria; OPG Count; Sulfadimidine
Abbreviation
CSA: Central Statistical Authority; DZARC: Debre-zeit
Agricultural Research Center; EARO: Ethiopian Agricultural
Research Organization; FAO: Food and Agricultural Organization;
Kca: Kilo caloric; NOAH: National Office of Animal Health; OPG:
Oocyst Per gram feaces; Rpm: Revolution per minute; SPSS:
Statistical Package for Social Science; USA: United States of
America
Introduction
Poultry refers to all domestic birds such as chickens
(domestic fowl), turkeys, ducks, geese, guinea fowls, ostriches
and others, which are mainly kept for the production of meat
and egg for human consumption. Among these, chickens are the
most important species, adapted globally to various climatic
conditions where human being lives and play a significant role
in supplying animal origin protein to improve the nutrition of
human being [1].
In developing countries, the raising of chickens contributes an opportunity to feed the rapid growing of human population and generate income for poor farmers. In addition, poultry in several parts of the modern world is considered to be the chief source of animal origin protein and high quality for human consumption [2].
Population of poultry in Ethiopia estimated to be 42 million excluding pastoral and agro-pastoral areas [2]. Among those only 1% is raised under intensive management system while the remaining 99% are raised under back yard or traditional low input production system which losses revenues and food subsistence in the country [3]. Indigenous chickens in Ethiopia are predominantly raised where free range scavenging management system is practiced and thus birds are left to depend primarily on what nature offers [4]. On the other hand, the current trend indicates the improved breeds kept under small scale poultry farm especially in and around Addis Ababa, Bishoftu and Adama [5].
Diseases and management problems become extremely important both in the commercial and backyard production system. In rural Ethiopia the main constraints for the improvement of local poultry production systems are diseases, predation, malnutrition, housing and poor management system [6]. Chickens’ prevalent diseases in Ethiopia are Newcastle disease, Coccidiosis, Salmonellosis, chronic respiratory disease and nutritional deficiency. Poultry mortality due to disease is estimated between 20-50% but can go as high as 80% during time of epidemics. Among those diseases, the parasitic protozoan disease coccidiosis is economically important and prevalent in chicken farm in Ethiopia [3]. Parasitic diseases are of particular importance because of their high incidence in poultry occasioned by the tropical environmental conditions under which the farmer operates [7].
The disease of coccidiosis is caused by the Phylum Sporozoa, with few exceptions the organisms are intracellular parasites of intestinal epithelium [8]. The Coccidia occurring in poultry belongs almost exclusively to the genus Eimeria [9]. In chicken, coccidiosis is caused by nine species of Eimeria among which six species such as E.tenella, E. necatrix, E. brunetti, E. maxima, E. acervulina, and E. mivati produce moderate to severe intestinal or caecal lesions where as E. praecox, E. hagani and E. mitis do not produce significant lesions [7]. According to [12]; [13] nine species of Eimeria have been described in chickens; seven are currently recognized as valid: Eimeria acervulina, Eimeria praecox, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria tenella, Eimeria brunetti. While Eimeria tenella causes caecal coccidiosis, the rest are known to cause intestinal coccidiosis [12]. Among the nine (9) species of coccidian affecting the chickens, Eimeria tenella and Eimeria maxima are the most common species [14]. Coccidial infections in chicken causes considerable economic losses than in other domesticate birds and it is caused by the genus Eimeria and nine species are known to occur in chicken, which are widely distributed throughout the world [15]. Coccidiosis remains one of the major disease problems of poultry in spite of advances made in prevention and control through chemotherapy, management and nutrition [16].
In Ethiopia Coccidiosis is endemic, causing great economic losses particularly in young growing birds in all production system. Quantitative losses due to Coccidiosis in Ethiopia are not well documented, but [17] has reported that Coccidiosis contributes to 8.4% loss in profit in large scale farms and 11.86% loss in profit in small scale farms. The success of poultry rearing is mainly dependent on improved management, improved nutritional status and effective disease prevention and control measures [18].
A succession of chemical compounds has been introduced and has been crucial in the successful control of coccidiosis in the rapidly expanding poultry industries throughout the world. However, the emergence of resistance has been rapid and has limited the useful life of many chemical anticoccidial drugs. Although the speed at which resistance develop varies greatly between compounds, continuous use of anticoccidial drugs promotes the emergence of drug resistant strains of coccidian. Despite the economic significance of the disease no major research work has been done to determine the treatment response of poultry coccidiosis (to evaluate the drug efficacy) in Ethiopia. Therefore, the objective of this study was to assess and compare the efficacy of Amprolium and Sulfadimidine used for treatment of poultry coccidiosis.
In developing countries, the raising of chickens contributes an opportunity to feed the rapid growing of human population and generate income for poor farmers. In addition, poultry in several parts of the modern world is considered to be the chief source of animal origin protein and high quality for human consumption [2].
Population of poultry in Ethiopia estimated to be 42 million excluding pastoral and agro-pastoral areas [2]. Among those only 1% is raised under intensive management system while the remaining 99% are raised under back yard or traditional low input production system which losses revenues and food subsistence in the country [3]. Indigenous chickens in Ethiopia are predominantly raised where free range scavenging management system is practiced and thus birds are left to depend primarily on what nature offers [4]. On the other hand, the current trend indicates the improved breeds kept under small scale poultry farm especially in and around Addis Ababa, Bishoftu and Adama [5].
Diseases and management problems become extremely important both in the commercial and backyard production system. In rural Ethiopia the main constraints for the improvement of local poultry production systems are diseases, predation, malnutrition, housing and poor management system [6]. Chickens’ prevalent diseases in Ethiopia are Newcastle disease, Coccidiosis, Salmonellosis, chronic respiratory disease and nutritional deficiency. Poultry mortality due to disease is estimated between 20-50% but can go as high as 80% during time of epidemics. Among those diseases, the parasitic protozoan disease coccidiosis is economically important and prevalent in chicken farm in Ethiopia [3]. Parasitic diseases are of particular importance because of their high incidence in poultry occasioned by the tropical environmental conditions under which the farmer operates [7].
The disease of coccidiosis is caused by the Phylum Sporozoa, with few exceptions the organisms are intracellular parasites of intestinal epithelium [8]. The Coccidia occurring in poultry belongs almost exclusively to the genus Eimeria [9]. In chicken, coccidiosis is caused by nine species of Eimeria among which six species such as E.tenella, E. necatrix, E. brunetti, E. maxima, E. acervulina, and E. mivati produce moderate to severe intestinal or caecal lesions where as E. praecox, E. hagani and E. mitis do not produce significant lesions [7]. According to [12]; [13] nine species of Eimeria have been described in chickens; seven are currently recognized as valid: Eimeria acervulina, Eimeria praecox, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria tenella, Eimeria brunetti. While Eimeria tenella causes caecal coccidiosis, the rest are known to cause intestinal coccidiosis [12]. Among the nine (9) species of coccidian affecting the chickens, Eimeria tenella and Eimeria maxima are the most common species [14]. Coccidial infections in chicken causes considerable economic losses than in other domesticate birds and it is caused by the genus Eimeria and nine species are known to occur in chicken, which are widely distributed throughout the world [15]. Coccidiosis remains one of the major disease problems of poultry in spite of advances made in prevention and control through chemotherapy, management and nutrition [16].
In Ethiopia Coccidiosis is endemic, causing great economic losses particularly in young growing birds in all production system. Quantitative losses due to Coccidiosis in Ethiopia are not well documented, but [17] has reported that Coccidiosis contributes to 8.4% loss in profit in large scale farms and 11.86% loss in profit in small scale farms. The success of poultry rearing is mainly dependent on improved management, improved nutritional status and effective disease prevention and control measures [18].
A succession of chemical compounds has been introduced and has been crucial in the successful control of coccidiosis in the rapidly expanding poultry industries throughout the world. However, the emergence of resistance has been rapid and has limited the useful life of many chemical anticoccidial drugs. Although the speed at which resistance develop varies greatly between compounds, continuous use of anticoccidial drugs promotes the emergence of drug resistant strains of coccidian. Despite the economic significance of the disease no major research work has been done to determine the treatment response of poultry coccidiosis (to evaluate the drug efficacy) in Ethiopia. Therefore, the objective of this study was to assess and compare the efficacy of Amprolium and Sulfadimidine used for treatment of poultry coccidiosis.
Materials and Methods
Study area and population
The study was conducted in the poultry farm of Ethiopian
Agricultural research centre at Bishoftu. This poultry farm has
many breeds of chickens such as Local (Horo), Cross, Dominant,
Fayoumi, Kokock and Hubbard classic. The farm rears these
chickens in 10 different houses and it has its own hatchery and
incubation room. Bishoftu is located about 47 kilometers South
East of Addis Ababa. The area is located at about 1850 meters
above sea level. The town has the population of about 95,000
people. The soil and climate of the area are similar to those in
many highland areas in Ethiopia. The main rainy season of the
area ranges from June to September with an average rain fall of
800 millimeters, of which 84% of the rain is expected. There is
also a short rainy season from March to May. The annual average
temperature ranges from 12.3oc to 27.7oc with an average
temperature of 18.7oc. The highest temperature is reached in May
[19]. The study was carried out on different breeds of chickens
reared in six poultry houses at the Ethiopian Agricultural research
centre poultry farm. The study covered the breeds such as Cross,
Dominant, Fayoumi, Horo and Kokock with their numbers 6, 6,
12, 14 and 14 respectively.
Study Design, Sampling and Methods
An experimental type of study design was conducted to assess
the treatment response of the Coccidiosis to the Amprolium and
Sulfadimidine drugs. Chickens used in this experiment were
selected purposively. The study was conducted on the freshly
collected fecal sample from each control and treatment group
(before treatment and after treatment with Amprolium and
Sulfadimidine) of chickens. In the study 35 chickens were used
for drugs treatments as treatment group, 18 of them were treated
with Amprolium and 17 of them were treated with Sulfadimidine.
The rest of 17 Chickens were used as control group. Fecal Oocyst
count (OPG count) was conducted as indicated [20] by using
modified McMaster Oocyst count to compare the efficacy of the
Amprolium and Sulfadimidine. The OPG count was performed
before and after treatment to evaluate the efficacy of the drugs by
comparing the OPG count before and after treatment. Amprolium
and Sulfadimidine were administered after the chickens in each
pen were grouped into treatment and control groups. These
drugs were administered for the treatment group with drinking
water with the dose of 1gram per liter of water for Amprolium
and 3 milliliter per liter of water in case of Sulfadimidine and the
administration continued for 7 and 5 days respectively. The OPG
count after treatment was conducted after 7 days for Amprolium
and after 5 days for Sulfadimidine.
Data Analysis
The data was entered into computer using Excel spreadsheet.
It was then analyzed using SPSS soft ware. A chi-square test was
used to analyze the data. The results of this study were considered
as significant when a P value was less than 0.05.
Results
The mean of Fecal Oocysts count (OPG count) before treatment
and after treatment was 3288.08 and 1468.65 respectively (Table
1). There was statistically significant difference (P < 0.05) in OPG
count between before treatment and after treatment and OPG
count after treatment was significantly reduced.
The mean difference between OPG counts before and after treatment of Amprolium and Sulfadimidine were 4691.11 and 2915.00; 4126.47 and 454.12 respectively. Treatment of Amprolium before and after was statistically insignificant (p > 0.05), but treatment of Sulfadimidine before and after was statistically significant (p < 0.05) (Table 2).
The percentages of the study conducted on poultry breeds of Cross, Dominant, Fayoumi, Horo (Local), and Kokock were 26.9%, 1.5%, 11.5%, 26.9%, and 23.1% respectively (Table 3).
The mean difference between OPG counts before and after treatment of Amprolium and Sulfadimidine were 4691.11 and 2915.00; 4126.47 and 454.12 respectively. Treatment of Amprolium before and after was statistically insignificant (p > 0.05), but treatment of Sulfadimidine before and after was statistically significant (p < 0.05) (Table 2).
The percentages of the study conducted on poultry breeds of Cross, Dominant, Fayoumi, Horo (Local), and Kokock were 26.9%, 1.5%, 11.5%, 26.9%, and 23.1% respectively (Table 3).
Discussion
The current study was undertaken to assess and compare
the efficacy of Amprolium and Sulfadimidine in naturally
coccidia infected chickens in Ethiopian Agriculture research
center poultry farm. In this study, the modified McMaster Oocyst
count (OPG count) was used to compare the efficacy of drugs.
The mean of Fecal Oocysts count (OPG count) before treatment
and after treatment was 3288.08 and 1468.65 respectively.
There was statistically significant difference (P < 0.05) in OPG
Table 1: Comparison of OPG count before treatment and after treatment
|
Mean |
Total number of sample (N) |
Std. Deviation |
Std. Error |
Significance |
OPG count before treatment |
3288.08 |
52 |
6214.391 |
861.781 |
0.010 |
OPG count after treatment |
1468.65 |
52 |
3346.899 |
464.131 |
|
Each group contained 10 kids
Table 2: Comparison of OPG count before and after treatment between
Amprolium and Sulfadimidine
Drug used |
Before / after treated |
Mean |
N |
Std. deviation |
Std. Error |
significance |
Amprolium |
OPG count before treatment |
4691.11 |
18 |
8948.783 |
2109.248 |
0.283 |
|
OPG count after treatment |
2915.00 |
18 |
5039.440 |
187.807 |
|
Sulfadimidine |
OPG count before treatment |
4126.47 |
17 |
5013.994 |
1216.072 |
0.004 |
|
OPG count after treatment |
454.12 |
17 |
821.942 |
199.350 |
|
Table 3: Frequencies and percent of breeds used in the study
Breed |
Frequencies |
Percent (%) |
Cross |
14 |
26.9 |
Dominant |
6 |
1.5 |
Fayoumi |
6 |
11.5 |
Horo (local) |
14 |
26.9 |
Kokock |
12 |
23.1 |
Total |
52 |
100.00 |
count between before treatment and after treatment and OPG
count after treatment was significantly reduced. Report by [12]
Compare the efficacy of Diclazuril and Amprolium in naturally
infected broilers in Uganda. In that study they found that there
was a significant reduction (P < 0.05) by ANOVA in the mean OPG
counts of the treatment and control groups.
The mean difference between OPG counts before and after treatment of Amprolium and Sulfadimidine were 4691.11 and 2915.00; 4126.47 and 454.12 respectively. Treatment of Amprolium before and after was statistically insignificant (p > 0.05), but treatment of Sulfadimidine before and after was statistically significant (p < 0.05). Based on this, Sulfadimidine had higher efficacy than Amprolium. This could be due to the fact that Amprolium have been in use in this poultry farm over many years and resistance to it might have developed as it is claimed. On the other hand, even if sulfonamides were the primary drugs for the treatment of coccidiosis in many parts of the world; Sulfadimidine is a more recently used for the treatment of coccidiosis in this poultry farm and it is less likely to show resistance. Continuous use of anticoccidial drugs has led to ineffective treatment due to drug resistance in the target parasites population. Drug resistance is heritable and depends on the amount of Oocysts discharged from infected chickens depends on the dose of Oocysts ingested and the immunological status acquired from pre- exposure (re-infection) [21]. According to [22] some studies in the floor pens and the field indicate that chickens given various drug programmes can develop immunity when exposed to natural infections. The greater or lesser extent of interference of anticoccidial drugs is not barrier to the development of immunity to Eimeria species. Therefore, the acquisition of immunity is a real possibility as long as sufficient parasites are present in the environment. Eventhough, anticoccidial drugs over a long time may lead to emergence of drug resistant population of coccidian. For this matter, it is important to review or change the medicine used as coccidiosis start from time to time [1]. Drug resistance mechanism involves either difference in drug metabolism within the parasite or mutations at the binding site of the drugs [23]. As indicated in the previous study Amprolium and Sulfaquinoxaline which is sulfonamide group have been used intensively in Nigeria for over two decades and there have been a development of resistance to the two drugs in the field [24]. There was also a study conducted in Nigeria by [25] to compare the efficacy of Toltrazuril, Amprolium and Embazine Fort. In that study, Toltrazuril had higher efficacy than Amprolium and Embazine Fort. The reason set for that was Amprolium and Embazine Fort have been in use in Nigeria for over two decades and there had been a development of resistance to the drugs, while Toltrazuril was a more recently introduced drug to Nigeria and was therefore less likely to show resistance. [22] Had also reported wide spread of resistance to most drugs in use today including Amprolium and Sulfonamides.
The control of avian coccidiosis mainly relies almost entirely on chemotherapy, as it is evidenced from the fact that most intensively reared chickens are fed an anticoccidial agent in the diet throughout their growth periods. Feed or water medication is a convenient and cost effective method of enabling large numbers of chickens to be reared under intensive conditions. The practice of including drugs in the feed or water throughout the life of the bird has ensured that few parasites escape the effect of medication. In such an environment parasites are exposed throughout their life cycle to agents designed for their removal and this has inevitably resulted in the development of resistance [23].
It has been shown that parasites resistant to the recommended levels of certain anticoccidial drugs may be suppressed if the concentration of drug is increased. Resistance to this higher concentration, however, is likely to develop rapidly after further selection. Increasing the concentration of a drug may therefore, be the only use in the short term and in any case, would not be practical because most anticoccidial drugs are used at a level close to those that are toxic to the chicken. But, resistant strains may emerge if anticoccidial drugs have been used at concentrations lower than those normally recommended for control [23].
The mean difference between OPG counts before and after treatment of Amprolium and Sulfadimidine were 4691.11 and 2915.00; 4126.47 and 454.12 respectively. Treatment of Amprolium before and after was statistically insignificant (p > 0.05), but treatment of Sulfadimidine before and after was statistically significant (p < 0.05). Based on this, Sulfadimidine had higher efficacy than Amprolium. This could be due to the fact that Amprolium have been in use in this poultry farm over many years and resistance to it might have developed as it is claimed. On the other hand, even if sulfonamides were the primary drugs for the treatment of coccidiosis in many parts of the world; Sulfadimidine is a more recently used for the treatment of coccidiosis in this poultry farm and it is less likely to show resistance. Continuous use of anticoccidial drugs has led to ineffective treatment due to drug resistance in the target parasites population. Drug resistance is heritable and depends on the amount of Oocysts discharged from infected chickens depends on the dose of Oocysts ingested and the immunological status acquired from pre- exposure (re-infection) [21]. According to [22] some studies in the floor pens and the field indicate that chickens given various drug programmes can develop immunity when exposed to natural infections. The greater or lesser extent of interference of anticoccidial drugs is not barrier to the development of immunity to Eimeria species. Therefore, the acquisition of immunity is a real possibility as long as sufficient parasites are present in the environment. Eventhough, anticoccidial drugs over a long time may lead to emergence of drug resistant population of coccidian. For this matter, it is important to review or change the medicine used as coccidiosis start from time to time [1]. Drug resistance mechanism involves either difference in drug metabolism within the parasite or mutations at the binding site of the drugs [23]. As indicated in the previous study Amprolium and Sulfaquinoxaline which is sulfonamide group have been used intensively in Nigeria for over two decades and there have been a development of resistance to the two drugs in the field [24]. There was also a study conducted in Nigeria by [25] to compare the efficacy of Toltrazuril, Amprolium and Embazine Fort. In that study, Toltrazuril had higher efficacy than Amprolium and Embazine Fort. The reason set for that was Amprolium and Embazine Fort have been in use in Nigeria for over two decades and there had been a development of resistance to the drugs, while Toltrazuril was a more recently introduced drug to Nigeria and was therefore less likely to show resistance. [22] Had also reported wide spread of resistance to most drugs in use today including Amprolium and Sulfonamides.
The control of avian coccidiosis mainly relies almost entirely on chemotherapy, as it is evidenced from the fact that most intensively reared chickens are fed an anticoccidial agent in the diet throughout their growth periods. Feed or water medication is a convenient and cost effective method of enabling large numbers of chickens to be reared under intensive conditions. The practice of including drugs in the feed or water throughout the life of the bird has ensured that few parasites escape the effect of medication. In such an environment parasites are exposed throughout their life cycle to agents designed for their removal and this has inevitably resulted in the development of resistance [23].
It has been shown that parasites resistant to the recommended levels of certain anticoccidial drugs may be suppressed if the concentration of drug is increased. Resistance to this higher concentration, however, is likely to develop rapidly after further selection. Increasing the concentration of a drug may therefore, be the only use in the short term and in any case, would not be practical because most anticoccidial drugs are used at a level close to those that are toxic to the chicken. But, resistant strains may emerge if anticoccidial drugs have been used at concentrations lower than those normally recommended for control [23].
Conclusion and Recommendations
1. The current study concluded that Sulfadimidine is the
better option for the treatment of poultry coccidiosis than
Amprolium in the study area. Commonly coccidiosis is
treated with water soluble drugs such as Sulphonamides,
Amprolium, Diclazuril and Toltrazuril. This water
medication is convenient and can be rapidly given. The
resistant strains of coccidia may emerge if anticoccidial
drugs have been used at a concentration lower than
those normally recommended for use. The importance
of the use of anticoccidial drugs to which resistance is
not developed have been emphasizing in the present
experiment. Therefore, based on the above conclusions,
the following recommendations are forwardedRational
use of anticoccidial drugs in the treatment of chickens to
reduce the development of drug resistance.
2. Further studies with better facilities and larger area coverage should be conducted.
3. Immunization activities against chickens’ coccidiosis should be done.
4. In any poultry farm there should be strict biosecurity measures.
2. Further studies with better facilities and larger area coverage should be conducted.
3. Immunization activities against chickens’ coccidiosis should be done.
4. In any poultry farm there should be strict biosecurity measures.
Acknowledgment
We authors thanks Bishoftu research Center for their
assistance during this study.
Conflict Of Interest
We authors Declare that no interest conflict.
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