Research Article
Open Access
Significance of CD30 Expression by Epidermotropic T
Cells in Lymphomatoid Papulosis and Lymphomatoid
Pityriasis Lichenoides
Eric C Vonderheid1*, Marshall E Kadin2, Gladys H Telang3
1Adjunct Professor, Sidney Kimmel Cancer Center, Johns Hopkins Medical Institutions, Baltimore, USA
2Professor, Department of Dermatology, Boston University and Roger Williams Medical Center, Providence, RI, USA
3Associate Professor, Department of Dermatology, The Warren Alpert Medical School of Brown University, RI, USA
2Professor, Department of Dermatology, Boston University and Roger Williams Medical Center, Providence, RI, USA
3Associate Professor, Department of Dermatology, The Warren Alpert Medical School of Brown University, RI, USA
*Corresponding author: Eric Vonderheid, MD, 37580 S. Desert Sun Drive, Tucson, AZ 85739, USA, Tel: +520-825-2699; E-mail:
@
Received: November 28, 2016; Accepted: January 27, 2017; Published: February 16, 2017
Citation: Vonderheid EC, Kadin ME, Telang GH (2017) Significance of CD30 Expression by Epidermotropic T Cells in Lymphomatoid Papulosis and Lymphomatoid Pityriasis Lichenoides. Clin Res Dermatol Open Access 4(2): 1-6. DOI: http://dx.doi.org/10.15226/2378-1726/4/2/00153
Abstract
Background: The T cell activation antigen CD30 is widely
used to differentiate lymphomatoid papulosis (LyP) from pityriasis
lichenoides. However, CD30 has recently been reported to be
expressed by CD8+ cells in the epidermis and dermis of some cases of
pityiasis lichenoides et varioliformis acuta (PLEVA).
Methods: This observation prompted us to review our experience with cases initially diagnosed as LyP or “lymphomatoid” pityriasis lichenoides for CD30 expression by epidermotropic T cells.
Results: CD30 expression by more than 20% of the epidermotropic T cells was observed in a group of 10 cases characterized clinically by self-healing papulonodules (two cases also with infiltrated plaques) and pathologically by atypical lymphocytes with cerebriform nuclei within the epidermis and dermal infiltrate.
Conclusions: CD30 can be expressed by either CD4+ or CD8+ cells in cases of LyP with epidermotropic T cells (so-called mixed LyP type A/B), but three of our cases appear to be examples of lymphomatoid CD30+ PLEVA. In addition, three cases had serologic manifestations suggestive of a lupus erythematosus-like autoimmune disorder. We hypothesize that CD30+ lymphocytes with cerebriform nuclei in some cases result in accumulation of benign activated CD8+ and CD4+ T cells rather than neoplastic cells.
Keywords: LYP (lymphomatoid papulosis); CD30; PLEVA (pityriasis lichenoides et varioliformis acuta); Lupus Erythematosus
Methods: This observation prompted us to review our experience with cases initially diagnosed as LyP or “lymphomatoid” pityriasis lichenoides for CD30 expression by epidermotropic T cells.
Results: CD30 expression by more than 20% of the epidermotropic T cells was observed in a group of 10 cases characterized clinically by self-healing papulonodules (two cases also with infiltrated plaques) and pathologically by atypical lymphocytes with cerebriform nuclei within the epidermis and dermal infiltrate.
Conclusions: CD30 can be expressed by either CD4+ or CD8+ cells in cases of LyP with epidermotropic T cells (so-called mixed LyP type A/B), but three of our cases appear to be examples of lymphomatoid CD30+ PLEVA. In addition, three cases had serologic manifestations suggestive of a lupus erythematosus-like autoimmune disorder. We hypothesize that CD30+ lymphocytes with cerebriform nuclei in some cases result in accumulation of benign activated CD8+ and CD4+ T cells rather than neoplastic cells.
Keywords: LYP (lymphomatoid papulosis); CD30; PLEVA (pityriasis lichenoides et varioliformis acuta); Lupus Erythematosus
Introduction
CD30, a lymphocyte activation antigen that is expressed by
neoplastic T cells in a subset of mycosis fungoides and primary
cutaneous CD30+ lymphoproliferative diseases, is widely used
to differentiate lymphomatoid papulosis (LyP) from pityriasis
lichenoides. [1, 2] Recently, Kempf and coworkers reported
13 unusual cases of the acute ulceronecrotic form of pityriasis
lichenoides, i.e., pityriasis lichenoides et varioliformis acuta
(PLEVA) or Mucha-Habermann disease, in which a large
proportion of lymphocytes within the epidermis and dermis
expressed CD30. [3] As is frequently observed in PLEVA lesions,
a large proportion of lymphocytes in the dermal infiltrate and
overlying epidermis of their cases expressed CD8 (median, 70%;
range 50 to 90%). However unlike typical PLEVA, CD30 was
co-expressed by these cells (median, 60%; range, 15 to 80%).
Other T cell markers including CD7 were present. Of note, CD3
was expressed by less than 50% of dermal lymphocytes in 3
of 7 specimens, often seen in mycosis fungoides. [4] Notably,
molecular or serologic evidence of parvovirus B19 infection was
found in a majority of their patients.
Kempf's paper prompted us to review our experience with patients initially diagnosed to have LyP or "lymphomatoid" pityriasis lichenoides for CD30 expression by epidermotropic T cells. We discovered that CD30 expression by more than 20% of the epidermotropic T cells identifies a heterogeneous group characterized clinically by self-healing papules and occasionally small nodules (two cases also with infiltrated plaques) and pathologically by atypical lymphocytes with convoluted or cerebriform nuclei (cerebriform lymphocytes) within the epidermis and dermal infiltrate. Three of our 10 patients may have CD30+ PLEVA although one patient (described below) also had specimens that were typical of LyP, an observation that supports the concept of a close pathogenic relationship exists between these conditions. [3, 5, 6] We also discovered a possible association of CD30+ cutaneous lymphoproliferative disorders with undeclared lupus erythematosus.
Kempf's paper prompted us to review our experience with patients initially diagnosed to have LyP or "lymphomatoid" pityriasis lichenoides for CD30 expression by epidermotropic T cells. We discovered that CD30 expression by more than 20% of the epidermotropic T cells identifies a heterogeneous group characterized clinically by self-healing papules and occasionally small nodules (two cases also with infiltrated plaques) and pathologically by atypical lymphocytes with convoluted or cerebriform nuclei (cerebriform lymphocytes) within the epidermis and dermal infiltrate. Three of our 10 patients may have CD30+ PLEVA although one patient (described below) also had specimens that were typical of LyP, an observation that supports the concept of a close pathogenic relationship exists between these conditions. [3, 5, 6] We also discovered a possible association of CD30+ cutaneous lymphoproliferative disorders with undeclared lupus erythematosus.
Methods
All cases were evaluated by one of us (ECV) for recurrent, often
crusted and/or purpuric self-healing papules or occasionally
small nodules (two cases also with infiltrated plaques) and
prior histopathologic evidence of atypical lymphocytes in the
dermal infiltrate, suggesting a diagnosis of LyP or lymphomatoid
pityriasis lichenoides. Skin biopsy specimens were bisected with
one-half of the specimen processed for routine histology and
one-half frozen for immunohistochemical studies that included
antibodies reactive against CD2, CD3, CD4, CD5, CD7, CD8, CD62L
and CD30. Only cases in which CD30 was expressed by more
than 20% of the epidermotropic T cells were selected (Table 1).
The percentage of positively labeled cells in the dermal infiltrate
was visually estimated. LyP specimens with epidermotropic
cerebriform lymphocytes and also atypical immunoblast-like
cells with vesiculated nuclei and prominent nucleoli, sometimes
resembling Reed-Sternberg cells, in the dermal infiltrate were
classified as a mixed type of LyP (LyP-A/B) or type C LyP (LyP-C)
depending on the density of atypical cells in the dermal infiltrate.
Six specimens were also studied for evidence of a dominant T cell
clone by polymerase chain reaction (PCR) for rearrangement of
the T cell receptor gamma chain (TCR-γ) chain gene.
Results
The clinical and pathologic findings of 10 cases (1 male, 9
females; ages 12 to 78 years) that fulfilled the selection criteria
are summarized in Table 1. Because of self healing papulonodules
with cerebriform lymphocytes in the epidermis, the differential
diagnosis included LyP, lymphomatoid pityriasis lichenoides
and “pityriasis lichenoides-like” mycosis fungoides.[7,8] The
specimen of patient 1, which was obtained from an infiltrated 2.3
cm plaque, is an example of borderline LyP-C/anaplastic large
cell lymphoma (ALCL). Specimens from patients 2 and 3 are
considered to be examples of CD4+ LyP-A/B. In 5 other cases, most
epidermotropic T cells were CD8+. The specimens of patients 5, 6
and 7 also had a substantial number of admixed CD4+ cells within
the epidermis. Because cerebriform lymphocytes were present
in the epidermis and dermis, the findings were considered to
be most consistent with LyP-A/B with a component of reactive
CD8+ cells although CD8+ LyP or CD8+ pityriasis lichenoides-like
mycosis fungoides were in the differential diagnosis.
Patient 6 illustrates that skin specimens obtained at different times can vary between pityriasis lichenoides and LyP. Prior to referral, two specimens showed characteristic features of pityriasis lichenoides with absent or rare CD30+ dermal cells and two other specimens showed features typical for LyP-A with many CD30+ cells in the dermis (Figures 1 and 2).
The papule from patient 7, who had co-existing patch phase mycosis fungoides, had typical histopathologic features of LyPA/ B with 20% CD30+ cells within the epidermis, but without CD30+ cells in the dermal infiltrate despite the presence of
Patient 6 illustrates that skin specimens obtained at different times can vary between pityriasis lichenoides and LyP. Prior to referral, two specimens showed characteristic features of pityriasis lichenoides with absent or rare CD30+ dermal cells and two other specimens showed features typical for LyP-A with many CD30+ cells in the dermis (Figures 1 and 2).
The papule from patient 7, who had co-existing patch phase mycosis fungoides, had typical histopathologic features of LyPA/ B with 20% CD30+ cells within the epidermis, but without CD30+ cells in the dermal infiltrate despite the presence of
Figure 1: Skin specimen of papule from patient 6 shows a perivascular
infiltrate in the upper dermis without involvement of the epidermis.
Large atypical lymphocytes are present in the infiltrate and many neutrophils
are within the vessel (Insert).
Figure 2: A stain for CD30 on the same specimen shown in Figure 1
confirmed the presence of scattered large CD30+ cells in the dermal infiltrate,
but not within the epidermis. This section also shows a dense
dermal infiltrate with a subepidermal vesicle.
Figure 3: Biopsy from a papule of patient 6 shows numerous medium
to large sized atypical lymphocytes, some with cerebriform nuclei, concentrated
along the basal layer of the epidermis, scattered apoptotic
keratinocytes, and a wedge-shaped dermal infiltrate composed of both
normal and atypical lymphocytes.
atypical immunoblast-like cells. Of interest, immunophenotyping
of a patch of mycosis fungoides done elsewhere also showed
CD8+ epidermotropic T cells (CD30 staining not performed).
Patient 8, who had been diagnosed to have LyP-A (histopathology only), presented with self-healing papules with histologic features of LyP-C, specifically a wedge-shaped infiltrate containing numerous large atypical cells with prominent nucleoli including Reed-Sternberg-like cells, many eosinophils and mitotic figures, extravasated erythrocytes, and margination of neutrophils in blood vessels (Table 1, Figure 4). The immunophenotype of these atypical cells was CD8+CD4- CD30+ indicating the possibility of aggressive primary cutaneous epidermotropic CD8+ cytotoxic T-cell lymphoma, also known as Berti’s lymphoma. [10, 11] The infiltrate was not angiocentric nor was vasculitis present, features characteristic of LyP-E. [12] Epidermotropic T cells were CD8+ and approximately one-half expressed of CD30. Although the patient’s disease responded well to low doses of methotrexate, her disease would abruptly recur when methotrexate doses were reduced. (Figure 5). Seven and one half years after initial presentation, the patient developed a 3 cm tumor on her palm. The biopsy specimen contained a dense nodular dermal infiltrate of morphologically similar cells as the initial specimen with 80% of cells expressing CD30. A diagnosis
Patient 8, who had been diagnosed to have LyP-A (histopathology only), presented with self-healing papules with histologic features of LyP-C, specifically a wedge-shaped infiltrate containing numerous large atypical cells with prominent nucleoli including Reed-Sternberg-like cells, many eosinophils and mitotic figures, extravasated erythrocytes, and margination of neutrophils in blood vessels (Table 1, Figure 4). The immunophenotype of these atypical cells was CD8+CD4- CD30+ indicating the possibility of aggressive primary cutaneous epidermotropic CD8+ cytotoxic T-cell lymphoma, also known as Berti’s lymphoma. [10, 11] The infiltrate was not angiocentric nor was vasculitis present, features characteristic of LyP-E. [12] Epidermotropic T cells were CD8+ and approximately one-half expressed of CD30. Although the patient’s disease responded well to low doses of methotrexate, her disease would abruptly recur when methotrexate doses were reduced. (Figure 5). Seven and one half years after initial presentation, the patient developed a 3 cm tumor on her palm. The biopsy specimen contained a dense nodular dermal infiltrate of morphologically similar cells as the initial specimen with 80% of cells expressing CD30. A diagnosis
Table 1: Cases with more than 20% CD30+ epidermotropic T cells.
Case |
A/R/G |
Duration; Clinical Examination; |
Epidermal Changes |
Dermal Changes |
Lymphocytes |
Immunohistochemistry |
TCR-γ |
Final DX |
1
|
52WF |
4 mo; Regressed Paps plus 2.3 cm PQ; |
Ep (many) |
Li, PV, I, PA, M |
Medium-large HC CLs and LVC |
ED: 100% CD2+3+4+8-5-7-62L-CD30+; |
Pos (SSCP) |
LyP-C/ |
2 |
50WF |
5 mo; |
U, Ep (many) |
Li, PV, Neu (v), EE, M |
Medium-large HC CLs plus LVC/RSC in dermis |
ED: 100% CD2+3+4+5+7+8-62L+, 50% CD30+; |
ND |
LyP-A/B |
3 |
34BF |
5 yr; |
U/N, A, Ep (many) |
PV, I, SC, PDE, Eos, EE, Vas |
CLs plus LVC in dermis |
ED: CD4 >> CD8, |
Neg (SSCP) |
LyP-A/B |
4 |
12 WF |
1 yr; |
A, P, Ep (many) |
Li, PV, PA, EE |
Small CLs plus few medium sized CLs in dermis |
E: CD8 > CD4, scattered CD30+;* |
Neg (DGGE) |
LyP-A/B |
5 |
16 WF |
5 mo; |
A, P, U, Ep (many) |
Li, PV, PDE, EE, Eos, Vas, PA |
Medium, occasional large HC CLs also in dermis |
ED: CD8 > CD4, |
Neg (SSCP) |
LyP-A/B |
6 |
35WF |
15 mo; |
U, Ep (many) |
Li, PV, EE, Neu (v) |
Medium-large CLs |
ED: CD8 > CD4, |
Pos (DGGE) |
LyP-A/B |
7 |
36 WM |
7 yr; Ulcerated & crusted Paps + PTs ≤ 5 cm‡; |
A, U, Ep (B, many) |
Li, PV, MF, Neu (v), EE |
Medium-large HC CLs plus LVC in dermis |
E: CD8 >> CD4, 20% CD30+; |
ND |
LyP-A/B |
8
|
78WF |
12 yr; |
U, Ep (focal?) |
Li, PV, MF, Eos, EE, Neu (v) |
Small CLs, LVC/RSC |
ED: CD8 >> CD4, > 50% CD30+; |
ND |
LyP-C |
9 |
77WF |
2 yr; |
U, VA, Ep (many) |
PV, Neu (v), EE |
Medium-large HC CLs |
ED: Weak CD4 & CD8§, CD30+; |
ND |
LyP-B§ |
10 |
59 WF |
5 yr; |
A, VA, NK, EE, Ep (Many, basalar) |
Li, W, EE, Eos |
Medium-large HC CLs also dermis |
ED: CD4 = CD8, many CD30+*; |
Neg (DGGE) |
See discussion |
Pap, papule (1-10 mm diameter); Nd, nodule (11-20 mm diameter); PT, patch; PQ, infiltrated plaque; ED, epidermis; D, dermis; A, acanthosis; Sp,
spongiosis; U, ulceration; NK, necrotic/apoptotic keratinocytes or epidermal necrosis; EE, erythrocyte extravasation; Ep, epidermotropism; Li,
lichenoid; ND, not determined; Neu (v), neutrophils in small vessels; P, parakeratosis; PDE, papillary dermal edema; PV, perivascular; I, interstitial;
SC, subcutaneous; PA, periadnexal; TCR-γ, T-cell receptor gamma chain rearrangement; VA, vacuolar alteration; Vas, lymphocytic vasculitis;
M, mitoses; CL, lymphocytes with cerebriform nuclei; HC, hyperchromatic; LVC, lymphocytes with vesiculated nuclei and prominent nucleoli;
RSC, Reed-Sternberg-like cells; >, greater than; >>, much greater than; < , less than; W, wiry collagen bundles; DGGE, denaturing gradient gel
electrophoresis; SSCP, single-stranded conformation polymorphism;
* Not indicated which T cell subset is expressing CD30
‡ Biopsy specimen of patch diagnostic for mycosis fungoides
# Subacute lupus erythematosus favored as diagnosis, but anti-SSA/Ro and anti-SSB/La antibodies negative.
§ Large cerebriform cells express CD2, CD3, CD5, CD7, CD62L, and CD30 but stain weakly for CD4 and CD8.
* Not indicated which T cell subset is expressing CD30
‡ Biopsy specimen of patch diagnostic for mycosis fungoides
# Subacute lupus erythematosus favored as diagnosis, but anti-SSA/Ro and anti-SSB/La antibodies negative.
§ Large cerebriform cells express CD2, CD3, CD5, CD7, CD62L, and CD30 but stain weakly for CD4 and CD8.
of secondary ALCL was rendered and local field radiotherapy was
given with good response. The patient died 5 months later, but
the specific cause of death is unknown.
A papule from patient 9 had no CD30+ cells in the dermal infiltrate and was diagnosed as LyP-B. The large intraepithelial T cells in this case expressed an unusual CD3+CD4dimCD8dimCD30+ immunophenotype. In addition these cells expressed CD2, variable CD5, CD7, and CD62L. The CD4 CD8 double positive phenotype has been observed in a few cases of LyP-A or LyP-C including one case with the 6p25.3 translocation [9, 14-15] and mycosis fungoides. [15] It is also possible that CD4+CD8+ neoplastic T cells differentiated from double negative precursor cells under influence of thymic-like hormones produced in vivo by keratinocytes. [16] However, CD1 was not expressed, a finding that argues against differentiation from immature thymocytes. Alternatively, stimulation of CD4+ neoplastic T cells in the epidermis might have induced expression of CD8. [17]
Finally, similar to patient 1, patient 10 presented with scattered self-healing 3 to 15 mm papulonodules along with two infiltrated plaques on the face and antecubital fossa. (Figures 6 and 7). A biopsy obtained from a papule 3 months before referral showed some vacuolar interface alteration and extravasated erythrocytes, but also lymphocytes with enlarged hyperchromatic and somewhat pleomorphic nuclei in the dermal infiltrate. (Figure 8) These atypical cells were CD30-negative and the tentative diagnosis was lymphomatoid pityriasis lichenoides.
This patient also had a prior history of urticarial vasculitis and photosensitivity, suggesting that the facial plaques were a manifestation of subacute lupus erythematosus although this could not be confirmed by serologic studies. The specimen taken from a small plaque on the thigh showed a band-like dermal infiltrate, vacuolar interface alterations with apoptotic keratinocytes, but also many small-medium sized cerebriform lymphocytes in the epidermis and superficial dermis that supported a diagnosis of mycosis fungoides (Figures 9 and 10). The epidermotropic cells consisted of a mixture of CD4+ and CD8+ cells, many of which expressed CD30 and scattered CD30+
A papule from patient 9 had no CD30+ cells in the dermal infiltrate and was diagnosed as LyP-B. The large intraepithelial T cells in this case expressed an unusual CD3+CD4dimCD8dimCD30+ immunophenotype. In addition these cells expressed CD2, variable CD5, CD7, and CD62L. The CD4 CD8 double positive phenotype has been observed in a few cases of LyP-A or LyP-C including one case with the 6p25.3 translocation [9, 14-15] and mycosis fungoides. [15] It is also possible that CD4+CD8+ neoplastic T cells differentiated from double negative precursor cells under influence of thymic-like hormones produced in vivo by keratinocytes. [16] However, CD1 was not expressed, a finding that argues against differentiation from immature thymocytes. Alternatively, stimulation of CD4+ neoplastic T cells in the epidermis might have induced expression of CD8. [17]
Finally, similar to patient 1, patient 10 presented with scattered self-healing 3 to 15 mm papulonodules along with two infiltrated plaques on the face and antecubital fossa. (Figures 6 and 7). A biopsy obtained from a papule 3 months before referral showed some vacuolar interface alteration and extravasated erythrocytes, but also lymphocytes with enlarged hyperchromatic and somewhat pleomorphic nuclei in the dermal infiltrate. (Figure 8) These atypical cells were CD30-negative and the tentative diagnosis was lymphomatoid pityriasis lichenoides.
This patient also had a prior history of urticarial vasculitis and photosensitivity, suggesting that the facial plaques were a manifestation of subacute lupus erythematosus although this could not be confirmed by serologic studies. The specimen taken from a small plaque on the thigh showed a band-like dermal infiltrate, vacuolar interface alterations with apoptotic keratinocytes, but also many small-medium sized cerebriform lymphocytes in the epidermis and superficial dermis that supported a diagnosis of mycosis fungoides (Figures 9 and 10). The epidermotropic cells consisted of a mixture of CD4+ and CD8+ cells, many of which expressed CD30 and scattered CD30+
Figure 4: Patient 8 presented in July 1989 with a 12 year history of
scattered 2 to 5 mm ulceronecrotic papules that would regress spontaneously
on the trunk and extremities.
Figure 5: Three years after presentation, patient 8 developed numerous
papules and crusted nodules that suddenly appeared when methotrexate
was withdrawn.
Figure 6: Patient 10 presented with erythematous 3 to 15 mm papulonodules
and small plaques in different stages of evolution on her thighs
and elsewhere.
Figure 7:Patient 10 also had 3 by 8 cm erythematous plaque on the
right mandibular area.
Figure 8:Specimen obtained from a papule of patient 10 shows an
interface dermatitis with vacuolar alteration of the basal layer of the
epidermis, and lymphocytes with large hyperchromatic nuclei in the
dermal infiltrate (Insert).
Figure 9:Specimen from a small plaque on the thigh of patient 10
(shown in figure 6) reveals a band-like lymphoid infiltrate in the upper
dermis and involvement of the overlying epidermis.
Figure 10:The epidermis shows apoptotic keratinocytes and atypical
medium to large sized lymphocytes with hyperchromatic nuclei. Eosinophils
and extravasated erythrocytes are also present.
cells were in the dermal infiltrate. Although mycosis fungoides
occurring in a patient with pre-existing lymphomatoid pityriasis
lichenoides (or LyP-B) seemed possible, in retrospect, this patient
may have had plaques of subacute lupus erythematosus with
epidermotropic cerebriform lymphocytes that mimic mycosis
fungoides as described by Magro.[18] Unfortunately no follow
up information is available to clarify the diagnosis.
In addition to patient 10, two other patients in this series (cases 2 and 6) had manifestations suspicious for a concomitant connective tissue disorder. Specifically, patient 2 had fatigue, arthralgias, positive antinuclear antibody (1:1280 titer, homogeneous pattern), elevated sedimentation rate, biologic false positive RPR, and thrombocytopenia, but no antibodies against double-stranded DNA, cardiolipin, SSA/Ro, SSB/La, and extractable nuclear antigens. Patient 6 also had fatigue, arthralgias, positive antinuclear antibody (1:640 titer, nucleolar and fine speckled pattern) and single-stranded DNA antibody tests, but negative double-stranded DNA antibody and other autoimmune serologies including anti-SSA/Ro and SSB/La antibodies.
In addition to patient 10, two other patients in this series (cases 2 and 6) had manifestations suspicious for a concomitant connective tissue disorder. Specifically, patient 2 had fatigue, arthralgias, positive antinuclear antibody (1:1280 titer, homogeneous pattern), elevated sedimentation rate, biologic false positive RPR, and thrombocytopenia, but no antibodies against double-stranded DNA, cardiolipin, SSA/Ro, SSB/La, and extractable nuclear antigens. Patient 6 also had fatigue, arthralgias, positive antinuclear antibody (1:640 titer, nucleolar and fine speckled pattern) and single-stranded DNA antibody tests, but negative double-stranded DNA antibody and other autoimmune serologies including anti-SSA/Ro and SSB/La antibodies.
Discussion
We herein report that a high proportion of CD30+
epidermotropic T cells with cerebriform nuclei may be
encountered in skin lesions of patients with CD4+ and CD8+
LyP. CD30+ epidermotropic T cells may also occur in PLEVA and
mycosis fungoides. [3, 19] It is conceivable that three of the LyPA/
B cases (patients 4 to 6) are examples of lymphomatoid CD30+
PLEVA, especially patient 6 whose lesions showed features of both
entities over time. Given that CD30 is an lymphocyte activation
antigen and that normal T cells when stimulated in vitro via CD3
or CD2 in the presence of phorbol esters can develop cerebriform
nuclei, [20] we hypothesize that CD30+ cerebriform lymphocytes
in some cases might be the result of accumulation of benign
stimulated CD8+ and CD4+ T cells rather than neoplastic cells.
Finally, it is noteworthy that 3 of our patients had clinical and
serologic manifestations suggestive of a lupus erythematosuslike
autoimmune disorder. This observation may be more than
fortuitous considering that chronic virus infections including
parvovirus B19 are suspected to trigger pityriasis lichenoides,
LyP, and lupus erythematosus. [3, 21-24]
We conclude that CD30 may be rarely expressed not only by CD8+ cells in some cases of PLEVA as reported by Kempf, [3] but also by epidermotropic T cells in lesions with histopathologic features of LyP. Our observation that one of our patients had both types of lesions suggests the possibility of a related histogenesis between LyP and CD30+ PLEVA, We demonstrate the variability of CD30 positivity in this heterogeneous population of selfhealing papulonodules. Furthermore, some patients with CD30+ epidermotropic T cells had manifestations of a lupus-like autoimmune disorder, the significance of which warrants further investigation.
We conclude that CD30 may be rarely expressed not only by CD8+ cells in some cases of PLEVA as reported by Kempf, [3] but also by epidermotropic T cells in lesions with histopathologic features of LyP. Our observation that one of our patients had both types of lesions suggests the possibility of a related histogenesis between LyP and CD30+ PLEVA, We demonstrate the variability of CD30 positivity in this heterogeneous population of selfhealing papulonodules. Furthermore, some patients with CD30+ epidermotropic T cells had manifestations of a lupus-like autoimmune disorder, the significance of which warrants further investigation.
Acknowledgement
Dr. Kadin receives support from the Drs. Martin and Dorothy
Spatz Charitable Foundation.
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