2Department of Anesthesiology and Critical Care, University College of Medical Sciences and Guru Tegh Bahadur Hospital, Delhi, India
Methods: A prospective, randomized, triple-blinded trial was conducted in a tertiary care teaching hospital in Delhi, India, over a 16-month period. A total of 60 patients were randomized into two groups of 30 each (8 mmHg and 12 mm Hg group). Outcome measures included intra-operative cardiopulmonary parameters, operating time, postoperative pain and morbidity, and, liver function tests. Minimally invasive intra-operative cardiovascular monitoring was used. Analysis was by Student’s t-test, ANOVA F-test and the Turkey procedure.
Results: Demographic parameters, operation time, conversion rate and postoperative hospital stay were similar in both the groups. Port placement at 8mmHg pressure was difficult; in 6, pressure was increased to 12 mmHg. No significant complications or mortality were observed. Low pressure PP was significantly better in terms of pain scores (at 3, 6, 12, 24 hours; p=0.01) number of additional doses of analgesic used (0.3±0.6 vs 1.0±0.74 mg tramadol; p=0.00), and, total extra dose of analgesic used (15±29.80 vs 50±37.14 mg tramadol; p=0.00). Some differences in favour of the low pressure group were observed for arterial to end-tidal CO2 gradient, serum bilirubin and serum alanine tranferase; however, no significant differences were observed in cardiopulmonary parameters or liver function tests.
Conclusions: Laparoscopic cholecystectomy is feasible and safe at 8 mmHg pressure with slight modification of technique, and is associated with less pain compared to surgery at 12 mmHg. Studies with compromised cardiopulmonary or hepatic reserve are required to better quantify the benefits.
Key words: Low Pressure Pneumoperitoneum; Standard Pressure Pneumoperitoneum; Laparoscopic Cholecystectomy; Pain, Operative Difficulty; Pulse Waveform Analysis
Several studies have compared the effects of reduced pressure (7-9 mm Hg) with standard pressure (12-15 mm Hg) during LC [7-11]. These studies illustrate the feasibility of low pressure PP, along with some advantages in terms of postoperative pain. However, it is still unclear whether changes in metabolic and physiologic parameters viz. liver function tests and cardiopulmonary parameters, have any clinical significance [11]. Joshipura and colleagues have performed the only randomized trial on the subject from our country; incidentally, they are also amongst the few to have commented on the operative challenges faced by reducing the pressure [12].
The present study was designed to compare low pressure and standard pressure pneumoperitoneum used during LC in terms of intra-operative parameters (cardiopulmonary, metabolic), operative difficulty, postoperative pain, consumption of analgesics and liver profile derangement.
Patients with a diagnosis of symptomatic uncomplicated gallstone disease, scheduled for elective laparoscopic cholecystectomy were enrolled in the study. Patients with deranged liver function tests (including serum alkaline phosphatase), history of chronic liver disease or liver malignancy, history of chronic alcohol intake, history of jaundice, cardiopulmonary disease, morbid obesity, choledocholithiasis, biliary obstruction, cholangitis, carcinoma gallbladder, a recent (< 3 weeks)or ongoing episode of acute gallstone pancreatitis or acute cholecystitis, ongoing pregnancy, bleeding diathesis, or upper abdominal surgical scars were excluded from the study.
After admission, the following information was recorded on the case record form – age, sex, weight, height, body mass index (BMI), pre-operative ultrasound findings (wall thickness, pericholecystic fluid, common duct anatomy and diameter, and number of calculi), and, liver function tests (serum total bilirubin, serum alanine aminotransferase, serum alkaline phosphatase).
The subjects were randomized using computergenerated random numbers into one of two groups depending on the insufflation pressure used – group 1 (LPLC or 8 mm Hg group), and, group 2 (HPLC or 12 mm Hg group). Allocation concealment was achieved by the sealed envelope technique. The envelopes were opened in the operating room by technical staff responsible for setting the insufflation pressure.
After overnight fasting, patients received premedication with oral diazepam 0.2 mg/kg 2 hours before surgery, and, a single dose of prophylactic antibiotic (intravenous cefazolin) at induction of anesthesia. Intra-operative monitoring included continuous lead II electrocardiography, pulse oximetry, capnography, and spirometry (S5 monitor, DatexOhmeda®, USA). Radial artery cannulation was performed after confirming adequate collateral flow in the ulnar artery by the modified Allen’s test. The arterial cannula was connected to a FloTracTM sensor and VigileoTM monitor (Edwards Lifesciences®, USA). This enabled minimally-invasive measurement of mean arterial pressure, heart rate, and, cardiac index, using a validated pulse waveform analysis [13, 14]. The principle involved is that aortic pulse pressure is proportional to stroke volume and inversely proportional to aortic compliance.
Following establishment of monitoring, an intravenous crystalloid infusion (Ringer’s lactate) was initiated at 10 mL/ kg/hour. Anesthesia was induced using intravenous fentanyl 1-2 μg/kg with intravenous propofol 1-2.5 mg/kg, and tracheal intubation was facilitated by intravenous vecuronium 0.1 mg/kg as muscle relaxant. Anesthesia was maintained with a mixture of O2 and N2 O along with isoflurane (1 ± 0.1 MAC). Intra-operative drug dosages, fluids, and FiO2 were titrated to maintain heart rate and blood pressure at ±20% of baseline values, and SpO2 greater than 97%. Intra-operative ventilation was standardized in both groups using volume-controlled mode initiated at a tidal volume of 8 mL/kg, I:E ratio of 1:2 and a respiratory rate of 10 breaths per minute. Keeping tidal volume constant, eucapnia (end-tidal CO2 =35-40 mmHg) was maintained by varying the respiratory rate.
Hemodynamic variables viz. mean arterial pressure; heart rate and cardiac index were recorded prior to induction of anesthesia (basal), post-induction, and then every 10 minutes following creation of pneumoperitoneum until completion of the procedure. Ventilatory parameters viz. end-tidal CO2, mean airway pressure, peak airway pressure, Sp O2, tidal volume, respiratory rate and I:E ratio were recorded postinduction following intubation, and at 10 minute-intervals until completion of the procedure. Arterial blood gases analysis was performed post-induction (baseline), and then at 20 minutes post-pneumoperitoneum. Muscle relaxation was reversed with intravenous neostigmine 0.05 mg/kg and atropine 0.02 mg/ kg. Ondansetron 8 mg and ranitidine was administered for postoperative nausea and vomiting.
An operating room technician set the insufflator to the pressure indicated in the sealed envelope, and covered the display of the insufflator with adhesive strips paper to effectively achieve triple blinding (surgeon, patient, and evaluator). Cholecystectomy was performed by any one of four surgeons, with a minimum experience of 200 cases each. The standard four-port technique was used; pneumoperitoneum was created by the open method and the pressure was set to the randomized value from the outset. Patients were placed in a reverse Trendelenberg position (30°) with 15° elevation of the right shoulder after introduction of the four ports. If the surgeon was unable to proceed further due to poor visualization, he/she requested for an increase in pressure. The pressure was increased by the same technician to 12 mm Hg (if 8 mm Hg earlier), or was kept at 12 mm Hg (‘sham’ increase). This ensured continuation of blinding throughout the procedure. Conversion to open surgery was done only after the surgeon was convinced that further dissection was not possible at the second pressure, or there was another anatomical/ technical difficulty in the case (during grasping of fundus, dissection of Calot’s triangle, clipping of duct or artery, separation from liver bed, or extraction of the organ). All such events and the time at which the difficulty encountered were recorded. Injuries to CBD, gall bladder perforation, eletrocautery burns to parietal wall/ viscera were recorded. Intra-operative outcome parameters recorded were operating time (laparoscopic time only), conversion to higher pressures, conversion to open surgery, reasons for conversion, intra-operative injuries or mortality, and use of additional ports.
Postoperative analgesia in the form of intramuscular diclofenac 1.25 mg/kg 8 hourly for 24 hours was administered to all patients. Patients requesting for additional pain reliefwere prescribed intravenous tramadol (50 mg). The numbers of additional doses of analgesic, as well as the total additional dose used were recorded. Postoperative pain was also assessed by a visual analogue pain scale (VAS) at 3 hours, 12 hours, 24 hours and 48 hours after surgery. Pain scores were graded from 0 (no pain) to 10 (unbearable pain). The patients were discharged 24 hours after surgery if the course was unremarkable. Liver function tests were repeated on postoperative day 1 and day 7. Postoperative parameters recorded were surgical site infection, bile leak, port site hernia, prolonged ileus > 24 hrs, peritonitis, re-operation, postoperative jaundice, or, death.
|
LPLC group |
SPLC group |
p value (significant if <0.05)* |
Age in years (mean) |
33.83±14.15 |
38.93±13.09 |
0.807 |
Sex ratio (male: female) |
3:27 |
3:27 |
1 |
BMI (kg/m2) |
22.89±4.18 |
22.53±3.76 |
0.983 |
Length of postoperative hospital stay (days) |
1.43±0.63 |
1.40±0.50 |
0.82 |
Operating time (minutes) |
60±17.76 |
58.87±21.08 |
0.823 |
Conversion rate |
10% (3/30)^ |
6.66% (2/30) |
0.177 |
Number of additional doses of analgesic |
0.30±0.60 |
1.00±0.74 |
0.000 |
Total extra dose of analgesic (mg of tramadol) |
15±29.80 |
50±37.14 |
0.000 |
In the SPLC group too, there was difficulty encountered at various stages (creation of pneumoperitoneum: 2 cases, grasping gallbladder fundus: 3 cases, dissection of Calot’s: 9 patients, clipping of cystic duct: 1 case, and, dissection of gall bladder from bed: 9 cases). Here, 28 cases were completed at 12 mm Hg. Two cases (7%) were converted to open cholecystectomy due to difficult anatomy and adhesions.
No significant intra- or postoperative complications were observed in either group. There were no significant differences between the groups in operating time, conversion rate (due to factors other than pressure), and, the length of postoperative hospital stay (Table 1).
Heart rate decreased significantly after induction and creation of pneumoperitoneum in both groups; a slight increase was seen with the duration of pneumoperitoneum, however, there was no significant difference between the two groups (p=0.714) (Table 2). In both the groups, mean arterial pressure (MAP) was reduced after induction but increased above the baseline after creation of pneumoperitoneum; however, there was no significant difference between the two groups (p=0.632). In both groups, cardiac index decreased after induction, but was seen to increase gradually with the duration of pneumoperitoneum. The increase was more in the standard pressure group, however, the difference was not statistically significant (p=0.730). The absolute values of all parameters were within the clinically normal range.
LPLC group |
SPLC group |
|||||
Heart rate (bpm) |
Mean Arterial Pressure (mm Hg) |
Cardiac index (L/min/m2) |
Heart rate (bpm) |
Mean Arterial Pressure (mm Hg) |
Cardiac index (L/min/m2) |
|
Basal |
84±9 |
90±13 |
4.4±1.0 |
85±12 |
91±14 |
4.2±1.4 |
Post-induction |
80±12 |
86±14 |
4.0±1.3 |
84±15 |
89±14 |
3.9±1.1 |
10 min# |
77±10 |
92±13 |
3.9±1.0 |
77±10 |
95±8 |
4.2±1.2 |
20 min# |
78±10 |
92±12 |
3.7±0.9 |
77±10 |
92±10 |
4.1±1.3 |
30 min# |
78±8 |
93±13 |
4.0±1.0 |
78±10 |
93±10 |
4.3±1.3 |
40 min# |
79±9 |
93±12 |
4.1±0.9 |
79±11 |
91±8 |
4.3±1.2 |
Arterial blood gas analysis revealed a rise in pO2 and pCO2 (Figure 1) levels after 20 minutes of PP, which were significant within the groups, but not between the two groups. These changes were paralleled by a significant fall in pH and base excess values (Figure 2) in both groups, with no significant differences between the groups (p>0.05). None of the changed values were of clinical relevance. While a minimal change in arterial bicarbonate (Figure 2) values was seen in the LPLC group, a slight increase (~1.8 meqL-1) was noted in the SPLC group, possibly due to the greater pCO2 levels in this group. The differences between the groups were statistically significant (p=0.030).
LPLC group |
SPLC group |
|||||
Serum total bilirubin (mg/dL) |
Serum alanine aminotranferase (IU/L) |
Serum alkaline phosphatase (U/L) |
Serum total bilirubin (mg/dL) |
Serum alanine aminotranferase (IU/L) |
Serum alkaline phosphatase (U/L) |
|
Pre-operative |
0.697±0.345 |
31.43±19.08 |
174.74±56.43 |
0.635±0.348 |
36.04±29.40 |
141.38±114.84 |
Postoperative day 1 |
0.765±0.349 |
51.65±29.71 |
169.39±46.88 |
0.939±0.369 |
63.41±41.02 |
145.88±74.28 |
Postoperative day 7 |
0.660±0.189 |
37.78±18.94 |
167.17±51.88 |
0.670±0.190 |
49.29±29.69 |
127.25±49.71 |
Cardiovascular changes in laparoscopic cholecystectomy occur due to anesthesia, CO2 PP, and patient positioning. In our patients, the degree of tilt, both in the reversed Trendelenberg position and the right-up position, was kept constant for all patients. At the start of PP, increased intraabdominal pressure (IAP) compresses blood out of the splanchnic vasculature, increases the venous return and preload, and hence there is increase in the mean arterial pressure (MAP), systemic vascular resistance (SVR) and pulmonary vascular resistance (PVR), accompanied with a decrease in cardiac output (CO) [6,18]. SVR elevation may also be due to increased plasma renin activity, increased antidiuretic hormone (ADH) production, and influence of the sympathetic system. Increased MAP is associated with elevated right atrial pressure (RAP) and pulmonary capillary wedge pressure (PCWP). Hypotension may occur in relatively hypovolemic patients, when increased IAP along with intermittent positive pressure ventilation (IPPV) compresses the inferior vena cava (IVC) and raises the intrathoracic pressure. The reverse Tredelenburg position also reduces venous return, leading to reduced CO and MAP. At lower IAP, cardiovascular changes are less pronounced. All the parameters have been shown to be reversible after peritoneal exsufflation without any residual effects [19].
Only a few studies have dealt with comparison of cardiopulmonary parameters at different pressures of PP [7, 8, 12, 20, 21]. Wallace et al in a prospective, randomized doubleblinded study of 40 patients showed that there were no significant differences in intraoperative heart rate or cardiac index, although the latter fell significantly soon after insufflation in both groups. The fall in cardiac index lasted longer (7 vs 2 minutes) and coincided with a slower rise in mean arterial pressure in the group with higher insufflation pressure. The authors felt that the hemodynamic effects of carbon dioxide are overshadowed by the mechanical effects of increased intra-abdominal pressure during laparoscopy [8]. Dexter et al found a moderate increase in heart rate after peritoneal insufflation which reached statistical significance for the high pressure group. The MAP also rose significantly in both groups. Stroke volume (SV) and cardiac output (CO) were reduced after PP in both groups, however, both SV and CO were significantly higher (p< 0.04) subsequently in the low pressure group [7]. In our patients, the values of MAP and CI do suggest a similar trend of reduction after PP, however, no significant effects of differential pressure is observed.
It can be inferred that these pathophysiological responses can be deleterious, especially in individuals with compromised hemodynamic status (ASA III and IV patients). Preventive measures like preoperative volume loading (10- 12 mL/kg) may prevent a decrease in CO that is induced by high IAP with reverse Tredelenburg position. In ASA III and IV patients, invasive hemodynamic monitoring or transesophageal echocardiography (TEE) may be necessary for optimal outcomes. The rate and pressure of CO2 insufflation may be reduced to prevent vasovagal and other dangerous cardiovascular responses [6].
Due to high solubility in blood, CO2 is rapidly absorbed during PP, inducing hypercapnia and acidosis. The end-tidal CO2 concentrations increase initially, however, CO2 tends toaccumulate in the body during prolonged surgery and due to high IAP. This can be reliably excreted only by compensatory hyperventilation, as the renal excretion of excessive H+ is a much slower response (3-5 days).[6,22] Despite increasing the tidal volume of ventilation, respiratory acidosis and increased CO2 excretion persists up to 1 hr postoperatively. In patients with compromised cardiopulmonary function, CO2 retention may reach dangerous levels.[22]
Increased IAP leads to increased intrathoracic pressure, restricting lung expansion and decreasing functional residual lung capacity by up to 50%. The peak and plateau airway pressures are increased, leading to increased ventilationperfusion mismatch and intrapulmonary shunting. This may worsen the risk for developing hypoxemia in patients of chronic obstructive pulmonary disease (COPD).[22,23] The anesthesiologist usually attempts to avoid hypercapnia and acidosis by increasing the tidal volume or by using positive end-expiratory pressure (PEEP), however, overuse of these maneuvers can cause ventilator-induced lung injury or further reduction of cardiac output, respectively. The routine use of other gases like argon, helium, etc., is still under study. Sefr et al found no significant differences in pH, pCO2, pO2, base excess, and HCO3- levels between the two groups randomized for different pressures of PP.[20] They concluded that PP negatively influences acid-base changes, however, whether these changes are more due to high IAP or transperitoneal CO2 absorption, is not clear. In a similar trial, Joshipura and colleagues found no significant changes in pCO2, total CO2, pH, base excess, and HCO3- levels. [12] They noticed differences in pO2 and end-tidal CO2 levels; however, we regard these parameters as modifiable and have not included them for objective comparisons. In a systematic review of randomized clinical trials comparing different pressures of PP in patients undergoing LC, Gurusamy and colleagues [11] found no differences in heart rate, mean arterial pressure, cardiac index, and arterial pH between the two groups. They concluded that there is insufficient information about the cardiopulmonary benefits of low pressure PP, and further trials are required, particularly inclusive of patients with higher ASA grades in terms of cardiopulmonary morbidity.
We have compared the mean arterial to end-tidal CO2 gradient (pCO2 (a-et)), which is a better indicator of ventilationperfusion mismatch in the lungs, and is expected to change due to basal lung compression in PP.[24] No previous study has dealt with this parameter.
Pain after laparoscopic cholecystectomy may be due to several mechanisms;[15] viz. the incision sites, peritoneal stretching, or, from the surgical trauma. Diaphragmatic irritation due to surgical drains or bile is also believed to play role, especially with regard to shoulder-tip pain.[9] Higher pressures of PP are believed to predispose to worse pain scores after LC. After adequate blinding of patients to eliminate bias, two studies report significant decrease in overall pain with low pressure PP.[8,9] Gurusamy et al, in their systematic review, believe the main advantage of low pressure PP is, in fact, decreased pain.[11] This is probably the only positive result in our study too.
We noticed transient elevation of serum bilirubin and serum alanine aminotransferase in both groups of patients, however, these elevations were not clinically or statistically significant. Retardation of hepatic blood flow by PP is postulated to cause elevation in liver enzymes, though not to clinically significant levels. [25] These changes have been shown to be independent of direct trauma to the liver. [26,27] Higher pressures during PP have been shown to produce more marked changes in liver function tests. [26,28] However, none of these studies have reported clinically significant changes. This leads to the conclusion that cautious use of higher PP pressure is prudent, especially when liver function is compromised.
- Gurusamy KS, Koti R, Samraj K, Davidson BR. Abdominal lift for laparoscopic cholecystectomy. Cochrane Database Syst Rev. 2012;5:CD006574. doi:10.1002/14651858.CD006574.pub2
- Menes T, Spivak H. Laparoscopy: searching for the proper insufflation gas. Surg Endosc. 2000;14(11):1050-1056.
- Neuhaus SJ, Gupta A, Watson DI. Helium and other alternative insufflations gases for laparoscopy. Surg Endosc. 2001;15(6): 553-560.
- Baraka A, Jabbour S, Hammoud R, Aouad M, Najjar F, Khoury G, et al. End-tidal carbon dioxide tension during laparoscopic cholecystectomy. Correlation with the baseline value prior to carbon dioxide insufflation. Anaesthesia 1994;49(4):304-306.
- Joris JL, Noirot DP, Legrand MJ, Jacquet NJ, Lamy ML. Hemodynamic changes during laparoscopic cholecystectomy. Anesth Analg. 1993;76(5):1067-1071.
- Henny CP, Hofland J. Laparoscopic surgery: pitfalls due to anesthesia, positioning, and pneumoperitoneum. SurgEndosc. 2005;19(9):1163-1171.
- Dexter SP, Vucevic M, Gibson J, McMahon MJ. Hemodynamic consequences of high- and low-pressure capnoperitoneum during laparoscopic cholecystectomy. Surg Endosc. 1999;13(4):376-381.
- Wallace DH, Serpell MG, Baxter JN, O'Dwyer PJ. Randomized trial of different insufflation pressures for laparoscopic cholecystectomy. Br J Surg. 1997;84(4):455-458.
- Barczynski M, Herman RM. A prospective randomized trial on comparison of low-pressure (LP) and standard-pressure (SP) pneumoperitoneum for laparoscopic cholecystectomy. SurgEndosc. 2003;17(4):533-538.
- Koc M, Ertan T, Tez M, Kocpinar MA, Kilic M, Gocmen E, et al. Randomized, prospective comparison of postoperative pain in low- versus high-pressure pneumoperitoneum. ANZ J Surg. 2005;75(8):693-696.
- Gurusamy KS, Samraj K, Davidson BR. Low pressure versus standard pressure pneumoperitoneum in laparoscopic cholecystectomy. Cochrane Database Syst Rev. 2009;2:CD006930.
- Joshipura VP, Haribhakti SP, Patel NR, Naik RP, Soni HN, Patel B, et al. A prospective randomized, controlled study comparing low pressure versus high pressure pneumoperitoneum during laparoscopic cholecystectomy. Surg Laparosc Endosc Percutan Tech. 2009;19(3):234-240.
- de Waal EE, Kalkman CJ, Rex S, Buhre WF. Validation of a new arterial pulse contour-based cardiac output device. Crit Care Med. 2007;35(8):1904-1909.
- Funk DJ, Moretti EW, Gan TJ. Minimally invasive cardiac output monitoring in the perioperative setting. Anesth Analg 2009;108(3):887-897. doi:10.1213/ane.0b013e31818ffd99
- Perrakis E, Vezakis A, Velimezis G, Savanis G, Deverakis S, Antoniades J, et al. Randomized comparison between different insufflation pressures for laparoscopic cholecystectomy. SurgLaparoscEndoscPercutan Tech. 2003;13(4):245-249.
- Chok KS, Yuen WK, Lau H, Fan ST. Prospective randomized trial on low-pressure versus standard-pressure pneumoperitoneum in outpatient laparoscopic cholecystectomy. SurgLaparoscEndoscPercutan Tech. 2006;16(6):383-386.
- Davides D, Birbas K, Vezakis A, McMahon MJ. Routine low-pressure pneumoperitoneum during laparoscopic cholecystectomy. SurgEndosc. 1999;13(9):887-889.
- O'Leary E, Hubbard K, Tormey W, Cunningham AJ. Laparoscopic cholecystectomy: haemodynamic and neuroendocrine responses after pneumoperitoneum and changes in position. Br J Anaesth. 1996;76(5):640-644.
- Gueugniaud PY, Abisseror M, Moussa M, Godard J, Foussat C, Petit P, et al. The hemodynamic effects of pneumoperitoneum during laparoscopic surgery in healthy infants: assessment by continuous esophageal aortic blood flow echo-Doppler. AnesthAnalg. 1998;86(2):290-293.
- Sefr R, Puszkailer K, Jagos F. Randomized trial of different intraabdominal pressures and acid-base balance alterations during laparoscopic cholecystectomy. SurgEndosc. 2003;17(6):947-950.
- Ibraheim OA, Samarkandi AH, Alshehry H, Faden A, Farouk EO. Lactate and acid base changes during laparoscopic cholecystectomy. Middle East J Anesthesiol. 2006;18(4):757-768.
- Hsieh CH. Laparoscopic cholecystectomy for patients with chronic obstructive pulmonary disease. J LaparoendoscAdvSurg Tech A. 2003;13(1):5-9.
- Rauh R, Hemmerling TM, Rist M, Jacobi KE. Influence of pneumoperitoneum and patient positioning on respiratory system compliance. J ClinAnesth. 2001;13(5):361-365.
- Choi DK, Lee IG, Hwang JH. Arterial to end-tidal carbon dioxide pressure gradient increases with age in the steep Trendelenburg position with pneumoperitoneum. Korean J Anesthesiol. 2012;63(3):209-215. doi:10.4097/kjae.2012.63.3.209
- Guven HE, Oral S. Liver enzyme alterations after laparoscopic cholecystectomy. J Gastrointestin Liver Dis. 2007;16(4):391-394.
- Morino M, Giraudo G, Festa V. Alterations in hepatic function during laparoscopic surgery. An experimental clinical study. SurgEndosc. 1998;12(7):968-972.
- Tan M, Xu FF, Peng JS, Li DM, Chen LH, Lv BJ, et al. Changes in the level of serum liver enzymes after laparoscopic surgery. World J Gastroenterol. 2003;9(2):364-367. doi: 10.3748/wjg.v9.i2.364
- Hasukic S. Postoperative changes in liver function tests: randomized comparison of low- and high-pressure laparoscopic cholecystectomy. Surg Endosc. 2005;19(11):1451-1455.